Anti-RANK antibody [64C1385] (ab13918)

Reviews (1)Q&A (6)References (3)

Overview

  • Product name
    Anti-RANK antibody [64C1385]
    See all RANK primary antibodies
  • Description
    Mouse monoclonal [64C1385] to RANK
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.

  • Positive control
    • WB: RAW (a mouse macrophage monocyte). IHC-P: Human T cell lymphoma and skin tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab13918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 97 kDa.
Flow Cyt Use 5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • Tissue specificity
    Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • Involvement in disease
    Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • Sequence similarities
    Contains 4 TNFR-Cys repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 265 antibody
      • CD265 antibody
      • FEO antibody
      • LOH18CR1 antibody
      • Loss of heterozygosity 18 chromosomal region 1 antibody
      • mRANK antibody
      • ODFR antibody
      • OFE antibody
      • OPTB7 antibody
      • Osteoclast differentiation factor receptor antibody
      • OSTS antibody
      • Paget disease of bone 2 antibody
      • PDB 2 antibody
      • PDB2 antibody
      • RANK antibody
      • Receptor activator of NF KB antibody
      • Receptor activator of NF-KB antibody
      • receptor activator of nuclear factor kappa B antibody
      • TNF receptor superfamily member 11a antibody
      • TNFRSF11A antibody
      • TNR11_HUMAN antibody
      • TRANCER antibody
      • Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody
      • Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody
      • Tumor necrosis factor receptor superfamily member 11A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)

      Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.

      See Abreview

    • Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)
      Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)

      Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)
      IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • He Y  et al. Retrospective investigation of "paint brush borders" sign in association with local recurrence of giant cell tumor of bone after intralesional curettage. J Bone Oncol 10:41-48 (2018). IHC-P ; Human . Read more (PubMed: 29255669) »
    • Ferreira CL  et al. Effect of orthodontic force associated with cigarette smoke inhalation in healthy and diseased periodontium. A histometric and immunohistochemistry analysis in rats. J Periodontal Res 53:924-931 (2018). Read more (PubMed: 30043971) »
    See all 3 Publications for this product

    Publishing research using ab13918? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANK antibody [64C1385]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Permeabilization
    Yes - saponine
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2014

    Question

    Dear Supplier,

    Please see customers inquiry below:

    For the following antibodies:


    RUNX2 Polyclonal Ab Cat#: ab102712

    RANKL Monoclonal Ab Cat#:ab45039

    RANK Monoclonal Ab Cat#: ab13918

    BMP-7 Polyclonal Ab Cat#:ab56023

    Osteoprotegerin (OPG) Polyclonal Ab Cat#:ab73400

    They are all compatible with IHC. Can you tell me if they will also work for FACS (Runx2; BMP-7 and OPG)?

    Looking forward for your response.

    Thanks

    Kind regards,

    Read More
    Answer

    Thank you for your enquiry.

    All tested applications and species covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    Therefore, I can confirm that RANK antibody ab13918 is indeed tested and covered by the guarantee for both IHC-P and flow cytometry.

    RUNX2 antibody ab102712
    This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

    The following RUNX2 antibody is tested in flow cytometry, I hope this may be of interest to your customer. However, please note this has not been tested in IHC and you will also need to check which species the customer is using. This has been tested and covered by the guarantee for human samples only.

    ab135674 RUNX2 antibody
    Tested in: Flow Cyt, WB
    Reacts with: Human
    https://www.abcam.com/index.html?datasheet=135674 (or use the following: https://www.abcam.com/index.html?datasheet=135674).


    RANKL antibody ab45039
    This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

    We do have the following RANKL antibodies tested in flow cytometry. Again, please note that these are not tested in IHC and please also check the species the customer is using:

    ab12125 RANKL antibody [12A380]
    Tested in: Flow Cyt, WB
    Reacts with: Human, Mouse
    https://www.abcam.com/index.html?datasheet=12125 (or use the following: https://www.abcam.com/index.html?datasheet=12125).

    ab95720 RANKL antibody [IK22/5] (Biotin)
    Tested in: Flow Cyt
    Reacts with: Mouse
    https://www.abcam.com/index.html?datasheet=95720 (or use the following: https://www.abcam.com/index.html?datasheet=95720).

    ab93553 RANKL antibody [IK22/5] (Phycoerythrin)
    Tested in: Flow Cyt
    Reacts with: Mouse
    https://www.abcam.com/index.html?datasheet=93553 (or use the following: https://www.abcam.com/index.html?datasheet=93553).

    BMP-7 antibody ab56023
    This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

    We do have the following BMP-7 antibody tested in flow cytometry. Again, please note that this is unfortunately not tested in IHC and please also check the species the customer is using:

    ab84030 BMP7 antibody (Biotin)
    Tested in: ELISA, Flow Cyt, sELISA, WB
    Reacts with: Human, Chinese Hamster
    https://www.abcam.com/index.html?datasheet=84030 (or use the following: https://www.abcam.com/index.html?datasheet=84030).

    Osteoprotegerin (OPG) antibody ab73400
    This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

    The following Osteoprotegerin antibody is tested in both IHC-P and flow cytometry. Please also check the species the customer is using to ensure it is suitable:

    ab105935 Osteoprotegerin antibody [ 5G2]
    Tested in: ELISA, Flow Cyt, ICC, ICC/IF, IHC-P, WB
    Reacts with: Human
    https://www.abcam.com/index.html?datasheet=105935 (or use the following: https://www.abcam.com/index.html?datasheet=105935).

    If your customer would like to test any of these antibodies in a previously untested species or application, please do not hesitate to contact us again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

    Question

    What I get that you are suggesting is that I test this ab22106 antibody using a Western blot and it might work but what I need is an antibody that works for IHC. What is the homology % with the ab13918?

    Read More
    Answer

    Thank you for your response.

    The identities of ab13918 with canine were 81%, with 89% positives and some gaps. This is generally the absolute limit for what we consider to be effective. I chose to recommend ab22106 because it was created with a shorter immunogen which shared similar identies. However the differences between the canine and immunogen are at the very end residues whereas the the differences with ab13918 are scattered throughout.

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Question

    Hi, I need the antibody to work in human, rat and mouse and for flow cytometry and immunohistochemistry. Regards  

    Read More
    Answer

    Thank you for your reply. I am pleased to provide the following information: We have two RANK antibodies tested flow cytometry, human and mouse (not IHC or rat): ab45092 RANK antibody [9A725] (Phycoerythrin) Mouse monoclonal Tested applications: Flow Cyt Reacts with: Human, Mouse  Click here (or use the following: https://www.abcam.com/index.html?datasheet=45092). ab13918 RANK antibody [64C1385] Mouse monoclonal Applications: Flow Cyt, IP, WB Reacts with: Human, Mouse  Click here (or use the following: https://www.abcam.com/index.html?datasheet=13918). One antibody tested in IHC-P, mouse and human (not rat or flow cytometry):   ab45039 Anti-RANKL antibody [12A668] Mouse monoclonal Tested applications: ICC/IF, IHC-P, WB Reacts with: Human, Mouse Click here (or use the following: https://www.abcam.com/index.html?datasheet=45039). I am sorry to confirm we have no RANK antibodies tested in rat. Regrettably I am not able to find the correct rat sequence on the protein database sites, and so am not able to check the alignments with rat.  If you have access to the rat sequence, I can suggest to try alignment to the immunogen for these antibodies. We recommend alignment should be over 85% to predict that an antibody will detect in a different species. I am sorry we also do not have an antibody tested and guaranteed in both IHC and flow cytometry. If you would like to test one of the antibodies in one of these untested applications, we would be able to provide a testing discount offer. Please let me know if you are interested, and I will be pleased to forward the details. This also applies to the rat species. If the alignment is over 70% , we would be able to provide a testing discount for you to try in rat. I am sorry we do not have an antibody that is tested to meet all your requirements on this occasion, but I hope these ones will still be of interest to you.  If you have any further questions, or are interested in the testing discount, please do not hesitate to contact me.

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    Question

    Thank you for your assistance with this matter. Upon reflection of our attempts to use this antibody, we have decided that we would prefer to have a refund for this purchase.

    Read More
    Answer

    Thank you for your email. I have instructed our accounting department to issue a refund for your order for ab13918. If you have any questions regarding the refund, please contact accounts@abcam.com If I can be of additional assistance, just let me know. Have a nice weekend!

    Read More

    Question

    Here's the responses to your questions: *How much protein did you load? *20, 40, and 60ug protien *Did you use reducing conditions? *I don't know... *How were your samples prepared?* RAW cells lysed in RIPA buffer (with PMSF, aprotinin and NaOthovanadate), frozen, thawed and protein quantified using BioRad DC Protien assay, 20ug protien added 1:3 with sample buffer (with 5% beta-mercaptoethanol), boiled 10 minutes, cooled to room temp and loaded into the gel. GAPDH antibody was used as loading control for membrane blotting, using the same secondary antibody conditions etc. *What did you block with? *BSA *Is the secondary antibody working with other primaries? *Yes *Did you check that the protein transferred properly to the membrane? *Yes; membrane was stained with ponceau S after transfer. *Also, what was the purchase order number or Abcam reference number that was used when you ordered this antibody?* PO: 51474 / ref: 3vapc51471

    Read More
    Answer

    Thank you for your email and patience. I enquired with the originator of this antibody and evidently RANK is a tricky protein to detect and depending upon how the RAW cells were cultured, you may or may not see a signal. However, I would expect that with a dilution as high as 1:100 and incubation overnight at 4C (which you said that you tried) that you would see something. There have not been any problems with this lot reported, but I can offer to send a free of charge replacement vial from a newer batch. Alternatively, I can offer a refund or credit note. Please let me know how you would like to proceed.

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    Question

    We ordered your RANK antibody ab13918 back in July, and after alloquoting and freezing it, hadn't gotten around to using it until this past month. I've been trying to do Western analysis of RANK expression from RAW cell lysates, and have not gotten any expression with this antibody. I have tried concentrations from 1:100 to 1:1000 (1:500 = 2 ug / ml), have tried incubation times ranging from 1hr at room temp to overnight at 4 degrees (also tried 1 hr room temp, overnight 4 deg, followed by an additional hour at room temp). I have also tried both HRP- and Cy5- conjugated secondaries, at various concentrations. I am now extremely frustrated with this antibody and am considering purchasing another RANK antibody from another source. Can you offer any suggestions as to how to remedy this problem?

    Read More
    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab13918 in Western blotting. I do need a few more details in order to trouble-shoot this: How much protein did you load? Did you use reducing conditions? How were your samples prepared? What did you block with? Is the secondary antibody working with other primaries? Did you check that the protein transferred properly to the membrane? Also, what was the purchase order number or Abcam reference number that was used when you ordered this antibody? Thank you, and I look forward to hearing from you.

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