Anti-RANK antibody [64C1385] (ab13918)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-283167-anti-rank-antibody-64c1385-immunohistochemistry.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)"){kind=icon}
Key features and details
- Mouse monoclonal [64C1385] to RANK
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-RANK antibody [64C1385]
See all RANK primary antibodies -
Description
Mouse monoclonal [64C1385] to RANK -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Mouse
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Immunogen
Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.
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Positive control
- WB: RAW (a mouse macrophage monocyte). IHC-P: Human T cell lymphoma and skin tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
64C1385 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use a concentration of 1 - 2 µg/ml.
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| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. -
Tissue specificity
Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. -
Involvement in disease
Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8792 Human
- Entrez Gene: 21934 Mouse
- Omim: 603499 Human
- SwissProt: Q9Y6Q6 Human
- SwissProt: O35305 Mouse
- Unigene: 204044 Human
- Unigene: 6251 Mouse
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Alternative names
- CD 265 antibody
- CD265 antibody
- FEO antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)
Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.
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![Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-21413-anti-rank-antibody-64c1385-immunoprecipitation.gif)
Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-21412-anti-rank-antibody-64c1385-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (10)
ab13918 has been referenced in 10 publications.
- Maeda T et al. RANK-RANKL signalling pathway contributes to disease progression in cutaneous angiosarcoma: a case report with an immunohistochemical review and in vitro experiments. J Eur Acad Dermatol Venereol N/A:N/A (2020). PubMed: 32511804
- He Y et al. Retrospective investigation of "paint brush borders" sign in association with local recurrence of giant cell tumor of bone after intralesional curettage. J Bone Oncol 10:41-48 (2018). IHC-P ; Human . PubMed: 29255669
- Ferreira CL et al. Effect of orthodontic force associated with cigarette smoke inhalation in healthy and diseased periodontium. A histometric and immunohistochemistry analysis in rats. J Periodontal Res 53:924-931 (2018). PubMed: 30043971
- Chen X et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839
- Verma SK et al. Cell-surface phosphatidylserine regulates osteoclast precursor fusion. J Biol Chem 293:254-270 (2018). PubMed: 29101233
- González Díaz EC et al. Mineralized Biomaterials Mediated Repair of Bone Defects Through Endogenous Cells. Tissue Eng Part A 24:1148-1156 (2018). PubMed: 29368582
- Ganju A et al. Protein kinase D1 regulates subcellular localisation and metastatic function of metastasis-associated protein 1. Br J Cancer 118:587-599 (2018). PubMed: 29465084
- Xu L et al. Glycosylation status of bone sialoprotein and its role in mineralization. Exp Cell Res 360:413-420 (2017). Mouse . PubMed: 28958711
- Zhang J et al. UHMWPE wear particles and dendritic cells promote osteoclastogenesis of RAW264.7 cells through RANK-activated NF-?B/MAPK/AKT pathways. Int J Clin Exp Pathol 10:9400-9408 (2017). PubMed: 31966812
- Zhu J et al. Role of RANK and Akt1 activation in human osteosarcoma progression: A clinicopathological study. Exp Ther Med 13:2862-2866 (2017). PubMed: 28587351
