Anti-RANK antibody [64C1385] (ab13918)
Key features and details
- Mouse monoclonal [64C1385] to RANK
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-RANK antibody [64C1385]
See all RANK primary antibodies -
Description
Mouse monoclonal [64C1385] to RANK -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.
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Positive control
- WB: RAW (a mouse macrophage monocyte). IHC-P: Human T cell lymphoma and skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
64C1385 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. -
Tissue specificity
Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. -
Involvement in disease
Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8792 Human
- Entrez Gene: 21934 Mouse
- Omim: 603499 Human
- SwissProt: Q9Y6Q6 Human
- SwissProt: O35305 Mouse
- Unigene: 204044 Human
- Unigene: 6251 Mouse
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Alternative names
- CD 265 antibody
- CD265 antibody
- FEO antibody
see all
Images
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Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.
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Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.
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IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (23)
ab13918 has been referenced in 23 publications.
- Kisielowski K et al. Prognostic relevance of clinicopathological factors in sporadic and syndromic odontogenic keratocysts: A comparative study. Adv Clin Exp Med 32:245-259 (2023). PubMed: 36226690
- Qiao W et al. SARS-CoV-2 infection induces inflammatory bone loss in golden Syrian hamsters. Nat Commun 13:2539 (2022). PubMed: 35534483
- Sun W et al. Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction. Nat Commun 13:2899 (2022). PubMed: 35610255
- Liang Q et al. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Cell Death Dis 12:336 (2021). PubMed: 33795653
- Shalev M et al. PTPRJ promotes osteoclast maturation and activity by inhibiting Cbl-mediated ubiquitination of NFATc1 in late osteoclastogenesis. FEBS J 288:4702-4723 (2021). PubMed: 33605542