Key features and details
- Rabbit polyclonal to RANK
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RANK antibody
See all RANK primary antibodies
DescriptionRabbit polyclonal to RANK
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human RANK. NCBI Reference Sequence: NP_003830.1.
Database link: Q9Y6Q6
- WB: 293T and EC109 cell lysate. IHC-P: Human cerebellum tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityCaprylic Acid - Ammonium Sulfate precipitation
Our Abpromise guarantee covers the use of ab219097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 66 kDa.|
FunctionReceptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
Tissue specificityUbiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Involvement in diseaseDefects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
Sequence similaritiesContains 4 TNFR-Cys repeats.
- Information by UniProt
- CD 265 antibody
- CD265 antibody
- FEO antibody
All lanes : Anti-RANK antibody (ab219097) at 2 µg/ml
Lane 1 : EC109 whole cell lysate
Lane 2 : 293T whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 66 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded human cerebellum tissue sections labeling RANK with ab219097 at 20 µg/mL.
ab219097 has not yet been referenced specifically in any publications.