Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)
- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-RANKL antibody [12A668] - BSA and Azide free
See all RANKL primary antibodies -
DescriptionMouse monoclonal [12A668] to RANKL - BSA and Azide free
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Human
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Immunogen
Recombinant full length protein corresponding to Mouse RANKL aa 1-317.
Database link: O14788 -
Positive control
- IHC-P: Human lymph node and liver tissue. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: Human lymph node lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferAzide free
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number12A668
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab45039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). | |
| IHC-P | Use a concentration of 1 - 5 µg/ml. |
Target
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FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
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Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
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Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localizationCytoplasm; Secreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 8600 Human
- Entrez Gene: 21943 Mouse
- Omim: 602642 Human
- SwissProt: O14788 Human
- SwissProt: O35235 Mouse
- Unigene: 333791 Human
- Unigene: 249221 Mouse
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Alternative names
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)](https://www.abcam.com/ps/products/45/ab45039/Images/ab45039-212683-ab4503911.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)Paraffin embedded human lymph node tissue stained for RANKL with ab45039 at 5 µg/ml.
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![Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)](https://www.abcam.com/ps/products/45/ab45039/Images/RANKL-Primary-antibodies-ab45039-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)ICC/IF image of ab45039 stained HeLa cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45039, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
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![Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)](https://www.abcam.com/ps/products/45/ab45039/Images/ab45039.jpg)
Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 2 µg + transfected cell lysate
Predicted band size: 35 kDa
Observed band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)](https://www.abcam.com/ps/products/45/ab45039/Images/ab45039-212682-ab4503912.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)Paraffin embedded human liver tissue stained for RANKL with ab45039 at a 1/500 dilution. Peroxidase-conjugate and DAB chromogen. A 2 hour incubation at RT was used.
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![Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)](https://www.abcam.com/ps/products/45/ab45039/Images/ab45039-282855-ab4503914.jpg)
Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039)Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 0.5 µg/ml + human lymph node lysate in RIPA buffer at 35 µg
Predicted band size: 35 kDa
Additional bands at: 28 kDa (possible isoform)Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)
Protocols
References
This product has been referenced in:
- Ji B et al. Isoliquiritigenin blunts osteoarthritis by inhibition of bone resorption and angiogenesis in subchondral bone. Sci Rep 8:1721 (2018). Read more (PubMed: 29379010) »
- Ding LZ et al. Mangiferin inhibits apoptosis and oxidative stress via BMP2/Smad-1 signaling in dexamethasone-induced MC3T3-E1 cells. Int J Mol Med 41:2517-2526 (2018). Read more (PubMed: 29484386) »
Customer reviews and Q&As
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