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the last June our company Aurogene ordered from you the antibodies ab10962 goat polyclonal to Osteoprotegerin, ab23760 rabbit polyclonal to ATF4 and ab45039 mouse monoclonal |12A668| ro RANKL, that didn't work when used by our customer. In attachment we send you the WB images of these antibodies. The experiments were performed using lysates of bone cells that certainly synthesize proteins for which the above antibodies should be specific. As for the first 2, the customer has found a strong aspecificity, while for RANKL he has seen a single band, but the found molecular weight (65 kDa approximately) is much higher than expected (35 kDa). Please, could you substitute as soon as possible these antibodies?
Asked on Nov 14 2011
Thank you for your enquiry. Sorry to hear your customer's are having trouble with these antibodies. What species of bone cells were being tested? What dilution of primary antibody for each? Was any other antibody used successfully with these same samples? How much protein was loaded on the gel? What was the purchase order number or Abcam order reference number associated with these purchases? Thanks in advance for the additional information.
Answered on Nov 14 2011