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Hi, I need the antibody to work in human, rat and mouse and for flow cytometry and immunohistochemistry. Regards
Asked on Nov 23 2011
Thank you for your reply. I am pleased to provide the following information: We have two RANK antibodies tested flow cytometry, human and mouse (not IHC or rat): ab45092 RANK antibody [9A725] (Phycoerythrin) Mouse monoclonal Tested applications: Flow Cyt Reacts with: Human, Mouse Click here (or use the following: https://www.abcam.com/RANK-antibody-9A725-PE-ab45092.html). ab13918 RANK antibody [64C1385] Mouse monoclonal Applications: Flow Cyt, IP, WB Reacts with: Human, Mouse Click here (or use the following: https://www.abcam.com/RANK-antibody-64C1385-ab13918.html). One antibody tested in IHC-P, mouse and human (not rat or flow cytometry): ab45039 Anti-RANKL antibody [12A668] Mouse monoclonal Tested applications: ICC/IF, IHC-P, WB Reacts with: Human, Mouse Click here (or use the following: https://www.abcam.com/RANKL-antibody-12A668-BSA-and-Azide-free-ab45039.html). I am sorry to confirm we have no RANK antibodies tested in rat. Regrettably I am not able to find the correct rat sequence on the protein database sites, and so am not able to check the alignments with rat. If you have access to the rat sequence, I can suggest to try alignment to the immunogen for these antibodies. We recommend alignment should be over 85% to predict that an antibody will detect in a different species. I am sorry we also do not have an antibody tested and guaranteed in both IHC and flow cytometry. If you would like to test one of the antibodies in one of these untested applications, we would be able to provide a testing discount offer. Please let me know if you are interested, and I will be pleased to forward the details. This also applies to the rat species. If the alignment is over 70% , we would be able to provide a testing discount for you to try in rat. I am sorry we do not have an antibody that is tested to meet all your requirements on this occasion, but I hope these ones will still be of interest to you. If you have any further questions, or are interested in the testing discount, please do not hesitate to contact me.
Answered on Nov 23 2011