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our customer told us that a control can not solve her problems. It could be useful only for the difference between the expected and observed molecular weight (in our case relative to RANKL antibody), but also in that case she's sure that there was no problem in gel running and the molecular weights were indeed those identified by the standard, because after revealing RANKL they have also revealed actin, with the band showing the expected length. Moreover, your the use of a control does not resolve the problem of OPG and ATF4 aspecificity. For this reason, she has requested the substitution of items ab10962, ab23760, ab45039. Please could you let us know when you should ship us these antibodies in substitution? Our customer needs them urgently.
Asked on Nov 28 2011
Thanks for your reply. It seems that, as part of their own internal QC, the customer has already peformed the control I suggested. This is great as it does appear clear now that these 3 antibodies are not working as expected. I would be happy to request the replacement antibodies the customer asked for: 1 vial each of ab10962, ab23760, ab45039. So I can process the request, please let me know the purchase order number or Abcam order reference number associated with the initial order of these 3 antibodies. Thanks!
Answered on Nov 28 2011