Anti-RANKL antibody (ab216484)
Key features and details
- Rabbit polyclonal to RANKL
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RANKL antibody
See all RANKL primary antibodies -
Description
Rabbit polyclonal to RANKL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human RANKL aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14788 -
Positive control
- IHC-P: Human gastric carcinoma and Mouse spleen tissue. Flow Cyt (Intra): Raji cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes. |
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Flow Cyt (Intra) |
1/100.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes. |
Flow Cyt (Intra)
1/100. |
Target
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Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
Tissue specificity
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
Involvement in disease
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localization
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8600 Human
- Entrez Gene: 21943 Mouse
- Omim: 602642 Human
- SwissProt: O14788 Human
- SwissProt: O35235 Mouse
- Unigene: 333791 Human
- Unigene: 249221 Mouse
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Alternative names
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin embedded human gastric carcinoma tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse spleen tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
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Intracellular Flow Cytometry analysis of Raji cellslabeling RANKL using ab216484 at 1/100 for 30 minutes (green) compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab216484 has been referenced in 13 publications.
- Cheat B et al. NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis. Front Immunol 13:839929 (2022). PubMed: 35281020
- Lektemur Alpan A et al. Evaluation of the Effects of Favipiravir Combined with Vitamin C on Alveolar Bone in Rats. J Evol Biochem Physiol 58:430-440 (2022). PubMed: 35599639
- Jeon HH et al. The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study. Life (Basel) 12:N/A (2022). PubMed: 36013326
- Kurt Y & Özmen Ö Effects of Vitamin C on the Oral-Nasal Mucosal Damage Caused by Favipiravir in Old and Young Rats. Cureus 14:e28796 (2022). PubMed: 36225521
- Huang B et al. AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus. Front Physiol 12:697738 (2021). PubMed: 36632609