Key features and details
- Rabbit polyclonal to RANKL
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RANKL antibody
See all RANKL primary antibodies
DescriptionRabbit polyclonal to RANKL
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
- IHC-P: Human gastric carcinoma and mouse spleen tissues. FACS: Raji cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
Cellular localizationCytoplasm; Secreted and Cell membrane.
- Information by UniProt
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded human gastric carcinoma tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse spleen tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
Flow cytometry analysis of Raji cells labeling RANKL using ab216484 at 1/100 for 30 minutes (green) compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
ab216484 has been referenced in 3 publications.
- Pan BL et al. Decreased microRNA-182-5p helps alendronate promote osteoblast proliferation and differentiation in osteoporosis via the Rap1/MAPK pathway. Biosci Rep 38:N/A (2018). PubMed: 30413613
- Yu FY et al. TNF-a increases inflammatory factor expression in synovial fibroblasts through the toll-like receptor-3-mediated ERK/AKT signaling pathway in a mouse model of rheumatoid arthritis. Mol Med Rep 17:8475-8483 (2018). PubMed: 29693122
- Zhen R et al. Hepatocyte growth factor improves bone regeneration via the bone morphogenetic protein-2-mediated NF-?B signaling pathway. Mol Med Rep 17:6045-6053 (2018). PubMed: 29436622