Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 0.5 - 5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.|
|ELISA||Use a concentration of 0.5 - 1 µg/ml.|
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
- Information by UniProt
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
ab10590 staining RANTES in human endothelial cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using Triton, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab10590 at 2µg/ml for 1 hour at 25°C. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution. Counter stained with TO-PRO3.
ab10590 staining RANTES in Human liver cancer metastases by Immunohistochemistry (Frozen sections).
Tissue was fixed with paraformaldehyde, blocked with 5% serum for 1 hour at 24°C and permeabilized with Triton X-100. Samples were incubated with primary antibody (in PBS + 0.3% Triton X-100 + 1% BSA) for 3 hours at 24°C. An AlexaFluor®488-conjugated donkey anti-goat IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Muthuswamy R et al. NF-?B hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cells. Cancer Res 72:3735-43 (2012). IHC-Fr ; Human . Read more (PubMed: 22593190) »