Overview

  • Product name
  • Description
    Rabbit polyclonal to RANTES
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hRANTES (human RANTES)

  • Positive control
    • Recombinant human RANTES protein (ab9680) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
IHC-P Use a concentration of 2 µg/ml.

Target

  • Function
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificity
    T-cell and macrophage specific.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

Images

  • ab9679 staining RANTES in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6) and tris buffer (pH 8). Samples were incubated with primary antibody (2μg/ml) for 1 hour at 25°C. An undiluted HRP-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab9679 staining RANTES in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.0 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an AEC chromogen.

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.

    See Abreview

  • Formalin-fixed, paraffin-embedded mouse colon tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.

    See Abreview

References

This product has been referenced in:
  • Bai D  et al. The effect of down-regulation of CCL5 on lipopolysaccharide-induced WI-38 fibroblast injury: a potential role for infantile pneumonia. Iran J Basic Med Sci 21:449-454 (2018). Read more (PubMed: 29922423) »
  • Liu Y  et al. TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus. Front Immunol 8:651 (2017). IHC-P ; Human . Read more (PubMed: 28620393) »
See all 11 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Breast cancer
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 25 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Colon
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 23 2018

Abreviews
Application
ELISA
Sample
Human Recombinant protein (recombinant human RANTES was used for calibration)
Specification
recombinant human RANTES was used for calibration
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Sandwich (Capture)

Tatiana Karakasheva

Verified customer

Submitted Nov 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample
Human Tissue sections (esophagus)
Specification
esophagus
Permeabilization
No
Fixative
Formaldehyde

Tatiana Karakasheva

Verified customer

Submitted Oct 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jul 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Both Cit (pH6) and Tris (pH8) work well
Sample
Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Permeabilization
No
Fixative
Methacarn

Abcam user community

Verified customer

Submitted Jul 31 2013

Answer

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

The exception is the rabbit monoclonal ab108933 but it has not been characterized as well as ab10590, and I am concerned that, since it is raised against the C-terminus, it might not be able to recognize platelet RANTES, depending on how RANTES is processed by platelets. The following note from the UniProt entry for the protein mentions that leukocytes secrete an N-terminal fragment.

"N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells."

http://www.uniprot.org/uniprot/P13501

I hope this helps. Please let us know if you have any other questions.

Read More

Answer

Thank you for contacting us.
I have been in contact with the lab, I can confirm that ab9679 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the antibody has been re-dissolved in PBS. There is an error on the datasheet concerning the reconstitution in sterile water and we apologize for this and thank you for bringing this to our attention as it will allow us to update our datasheet.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for your enquiry.

For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a concentration of 5.0 - 10.0 ug/ml of this antibody was required. This may need some individual optimization.

The recombinant protein used in this assay is NAP-2 protein ab9556.
https://www.abcam.com/index.html?datasheet=9556 (or use the following: https://www.abcam.com/index.html?datasheet=9556).

For ab9679, Rabbit Anti Human RANTES, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 0.4 – 0.6 μg/ml of this antibodywas required. This may need some individual optimization.

The recombinant protein used in this assay, is Rantes protein ab9680.
https://www.abcam.com/index.html?datasheet=9680 (or use the following: https://www.abcam.com/index.html?datasheet=9680).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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