Anti-RANTES antibody (ab9679)
- Datasheet
- References (15)
- Protocols
Overview
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Product nameAnti-RANTES antibody
See all RANTES primary antibodies -
DescriptionRabbit polyclonal to RANTES
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Neutralising, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant hRANTES (human RANTES)
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Positive control
- Recombinant human RANTES protein (ab9680) can be used as a positive control in WB.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. | |
| Neutralising | Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required. | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
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Tissue specificityT-cell and macrophage specific.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 6352 Human
- Omim: 187011 Human
- SwissProt: P13501 Human
- Unigene: 514821 Human
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Alternative names
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody (ab9679)This image is courtesy of an anonymous Abreviewab9679 staining RANTES in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6) and tris buffer (pH 8). Samples were incubated with primary antibody (2μg/ml) for 1 hour at 25°C. An undiluted HRP-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody (ab9679)ab9679 staining RANTES in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.0 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an AEC chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody (ab9679)This image is courtesy of an annoymous AbreviewFormalin-fixed, paraffin-embedded human breast cancer tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody (ab9679)This image is courtesy of an annoymous Abreview.Formalin-fixed, paraffin-embedded mouse colon tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.
Datasheets and documents
References
This product has been referenced in:
- Zlotkowska A & Andronowska A Variable chemokine expression in porcine trophoblasts and endometrium during the peri-implantation period. Theriogenology 131:16-27 (2019). Read more (PubMed: 30928625) »
- Jin S et al. Activation of cGMP/PKG/p65 signaling associated with PDE5-Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia. Prostate 79:909-919 (2019). Read more (PubMed: 30958912) »
