Anti-RANTES antibody (ab9679)
Key features and details
- Rabbit polyclonal to RANTES
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: unknown
Overview
-
Product name
Anti-RANTES antibody
See all RANTES primary antibodies -
Description
Rabbit polyclonal to RANTES -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Highly pure (>98%) recombinant hRANTES (human RANTES)
-
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
Properties
-
Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
Target
-
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. -
Tissue specificity
T-cell and macrophage specific. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
see all
Images
-
Sandwich ELISA - Anti-RANTES antibody (ab9679)
ab9679 detecting RANTES by sandwich ELISA, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
Datasheets and documents
References (19)
ab9679 has been referenced in 19 publications.
- Fujimoto Y et al. Significant association between high serum CCL5 levels and better disease-free survival of patients with early breast cancer. Cancer Sci 111:209-218 (2020). PubMed: 31724785
- Zlotkowska A & Andronowska A Variable chemokine expression in porcine trophoblasts and endometrium during the peri-implantation period. Theriogenology 131:16-27 (2019). PubMed: 30928625
- Jin S et al. Activation of cGMP/PKG/p65 signaling associated with PDE5-Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia. Prostate 79:909-919 (2019). PubMed: 30958912
- Xiang P et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling. Prostate 79:1018-1031 (2019). PubMed: 31018021
- Dangaj D et al. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors. Cancer Cell 35:885-900.e10 (2019). PubMed: 31185212
