Question (44033) | Anti-RANTES antibody (ab9679)

Go to datasheet (ab9679)

Question

Hi,

I am looking for a neutralizing antibody for CXCL7, and I came across this product:Anti-NAP2 antibody (ab9554). I was wondering if this is indeed tested to show that is neutralizes CXCL7 and how much to use to block CXCL7. In addition, I was wondering if abcam sells the function CXCL7 protein.

Also I am looking for a neutralizing antibody for Rantes and found Anti-Rantes antibody (ab9679).I was wondering if this is indeed tested to show that is neutralizes RANTES in solution and how much to use to block the protein. Also, if you could tell me if abcam sells the functional protein form of RANTES that would be great.

Kind Regards,

Answer

Thank you for your enquiry.

For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a concentration of 5.0 - 10.0 ug/ml of this antibody was required. This may need some individual optimization.

The recombinant protein used in this assay is NAP-2 protein ab9556.
https://www.abcam.com/index.html?datasheet=9556 (or use the following: https://www.abcam.com/index.html?datasheet=9556).

For ab9679, Rabbit Anti Human RANTES, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 0.4 – 0.6 μg/ml of this antibodywas required. This may need some individual optimization.

The recombinant protein used in this assay, is Rantes protein ab9680.
https://www.abcam.com/index.html?datasheet=9680 (or use the following: https://www.abcam.com/index.html?datasheet=9680).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

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