Question (72301) | Anti-RANTES antibody (ab9679)

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Question

I am looking to purchase an Anti-RANTES antibody in order to look at RANTEs expression in human platelets. I looked at product ab10590 and ab9679. Would either of these for Immunfloresence, or would you recommend a different anti-RANTES antibody?

Answer

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

The exception is the rabbit monoclonal ab108933 but it has not been characterized as well as ab10590, and I am concerned that, since it is raised against the C-terminus, it might not be able to recognize platelet RANTES, depending on how RANTES is processed by platelets. The following note from the UniProt entry for the protein mentions that leukocytes secrete an N-terminal fragment.

"N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells."

http://www.uniprot.org/uniprot/P13501

I hope this helps. Please let us know if you have any other questions.

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