Western blot abreview for Anti-RANTES antibody

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/5000

Detection

Detection method
IF
Bands
Specific: 8 kDa Non-specific: 28, 38, 50, 60, 85, kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
Patient with known increased levels of RANTES on ELISA of muscle homogenate

Additional data

Additional Notes
Too many non-specific bands that are brighter than expected band, but not surprising given whole tissue lysate

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Submitted Jul 31 2013

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