Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Thermo Scientific Starting Block T20 Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Sample
Human Tissue sections (esophagus)
Specification
esophagus
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS+0.1%BSA+0.2%TritonX-100
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
In normal esophagus, RANTES is expressed in the basal and suprabasal epithelial layers. Low level staining in the dermal layer is immune cell-derived. Magnified 10x (top) and 40x (bottom).

Tatiana Karakasheva

Verified customer

Submitted Oct 24 2014

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