ELISA abreview for Anti-RANTES antibody

Excellent
Abreviews
Application
ELISA
Sample
Human Recombinant protein (recombinant human RANTES was used for calibration)
Specification
recombinant human RANTES was used for calibration
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Sandwich (Capture)

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: ECL anti-Rabbit IgG HRP-linked whole antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
ab9666 was used as capture antibody, 1:2000 dilution. HRP substrate: TMB Microwell Peroxidase Substrate System (KPL)

Tatiana Karakasheva

Verified customer

Submitted Nov 03 2015

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