- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-RANTES antibody
See all RANTES primary antibodies -
DescriptionRabbit polyclonal to RANTES
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
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Species reactivityReacts with: Mouse
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Immunogen
Highly pure (>98%) recombinant mRANTES (mouse RANTES)
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. | |
| Neutralising | Use a concentration of 6 - 8 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mRANTES (100 ng/ml), |
Target
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FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
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Tissue specificityT-cell and macrophage specific.
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Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 20304 Mouse
- SwissProt: P30882 Mouse
- Unigene: 284248 Mouse
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Alternative names
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
see all
Protocols
References
This product has been referenced in:
- Nishikawa T et al. Systemic administration of platelets incorporating inactivated Sendai virus eradicates melanoma in mice. Mol Ther 22:2046-55 (2014). Read more (PubMed: 25023327) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"