Anti-RANTES antibody (ab9915)

Reviews (1)Q&A (1)References (1)

Overview

  • Product name
    Anti-RANTES antibody
    See all RANTES primary antibodies
  • Description
    Rabbit polyclonal to RANTES
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mRANTES (mouse RANTES)

  • Positive control
    • Recombinant mouse RANTES protein (ab9916) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use a concentration of 6 - 8 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mRANTES (100 ng/ml),

Target

  • Function
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificity
    T-cell and macrophage specific.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Beta chemokine RANTES antibody
      • Beta chemokine RANTES precursor antibody
      • C C motif chemokine 5 antibody
      • CCL 5 antibody
      • CCL5 antibody
      • CCL5_HUMAN antibody
      • Chemokine (C C motif) ligand 5 antibody
      • Chemokine CC Motif Ligand 5 antibody
      • D17S136E antibody
      • EoCP antibody
      • Eosinophil chemotactic cytokine antibody
      • MGC17164 antibody
      • RANTES(4-68) antibody
      • Regulated upon activation normally T expressed and presumably secreted antibody
      • SCYA 5 antibody
      • SCYA5 antibody
      • SIS delta antibody
      • SIS-delta antibody
      • SISd antibody
      • Small inducible cytokine A5 (RANTES) antibody
      • Small inducible cytokine A5 antibody
      • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
      • Small-inducible cytokine A5 antibody
      • T cell specific protein p288 antibody
      • T cell specific RANTES protein antibody
      • T cell-specific protein P228 antibody
      • T-cell-specific protein RANTES antibody
      • TCP 228 antibody
      • TCP228 antibody
      see all

    References

    This product has been referenced in:
    • Nishikawa T  et al. Systemic administration of platelets incorporating inactivated Sendai virus eradicates melanoma in mice. Mol Ther 22:2046-55 (2014). Read more (PubMed: 25023327) »
    See 1 Publication for this product

    Publishing research using ab9915? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RANTES antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6 and Tris/EDTA pH9
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - Triton x-100, 0.02% with serum block and primary antibody
    Fixative
    Paraformaldehyde
    Read More

    Ariel Stock

    Verified customer

    Submitted Dec 22 2014

    Question

    Dear Sir/Madam,

    I hope that you are fine and well on receiving this message. I would like to order a antibodies for detection of bovine CCL5 (RANTES) and GCP-2 (CXCL6) using ELISA. We would like to develop our own ELISA detection and therefore would like to know what primary (capture) antibody and secondary (detection) antibody could be used for this purpose. I have looked at your website and found some antibodies but unsure whether it would detect bovine sequences. Some of the antibodies stated that they detected either human, mouse or rat.

    If you could suggest appropriate antibodies for the described purpose, then it would be much appreciated.

    I look forward to your reply.

    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    The protein sequence compatibility between human and mouse with bovine is about 80% which is very close for antibody cross reactivity. We unfortunately haven't tested any of the antibody with bovine CCL5 so I am sorry we are unable to confirm their suitability. However as the homology is higher and therefore possibility of cross reactivity so the anti human might react with Bovine also. We can suggest following antibodies as pair

    ab83135 as capture - ab9915 as detection antibody.
    ab83324 as capture antibody - ab71978 as detection (this ab need to be conjugated)

    CXCL6; the possible combinations could be

    ab109783 (capture) and ab83138 (detection)

    Please note that none of these antibodies are actually tested with the bovine samples. Our assumption about the Bovine cross reactivity is totally based on the 5age sequence homology between human and bovine.

    As these antibodies are not tested with Bovine sample so these are eligible for 1

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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