Key features and details
- Rabbit polyclonal to RANTES (Biotin)
- Suitable for: ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-RANTES antibody (Biotin)
See all RANTES primary antibodies
DescriptionRabbit polyclonal to RANTES (Biotin)
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Rat
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Rat RANTES was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271272. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat RANTES protein.
|WB||Use at an assay dependent concentration.|
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
- Information by UniProt
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
Western blot analysis of varying levels of Recombinant rat RANTES protein using ab271272 at 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
RANTES was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271272. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/wellof recombinant RANTES protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271272 has not yet been referenced specifically in any publications.