Key features and details
- Rabbit polyclonal to RANTES (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-RANTES antibody (Biotin)
See all RANTES primary antibodies
DescriptionRabbit polyclonal to RANTES (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Mouse
Highly pure (>98%) recombinant mouse RANTES
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Purification notesab83135 is sterile filtered.
Our Abpromise guarantee covers the use of ab83135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine RANTES.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
The detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
- Information by UniProt
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
To detect murine RANTES by Western Blot analysis ab83135 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is reducing conditions; Murine recombinant RANTES protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 murine recombinant RANTES protein.
To detect mouse RANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83135 is required. This biotinylated polyclonal antibody, in conjunction with a appropriate anti-RANTES antibody as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RANTES.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83135 has not yet been referenced specifically in any publications.