Overview

  • Product name
    Anti-Rap1 antibody [1D2-1C64]
  • Description
    Mouse monoclonal [1D2-1C64] to Rap1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant full length protein corresponding to Human Rap1 aa 1-184.
    Sequence:

    MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQ CMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQI LRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKS KINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL


    Database link: P62834

  • Positive control
    • HeLa, Hek293T, U2OS and mouse NIH 3T3 cell lysates, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
IP Use at 2 µg/mg of lysate.
ICC/IF 1/50 - 1/200.

Target

Images

  • All lanes : Anti-Rap1 antibody [1D2-1C64] (ab175329) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293T cell lysate
    Lane 3 : U2OS cell lysate
    Lane 4 : Mouse NIH 3T3 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa

  • Immunofluorescence analysis of formalin-fixed permeabilized HeLa cells, labeling Rap1 (green, left panel) using ab175329 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).

  • Western blot analysis on immunoprecipitation pellet from mouse NIH 3T3 cells. The antigen-antibody complex was formed by incubating 750 µg of NIH 3T3 whole cell lysate with 2 µg of ab175329 overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose, washes extensively and eluted in sample buffer. 1) 25 µg of NIH 3T3 whole cell lysate, as a control, and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175329 at a 1/500 dilution. Chemiluminescent detection was perfomed.

References

This product has been referenced in:
  • Ling X  et al. TERT regulates telomere-related senescence and apoptosis through DNA damage response in male germ cells exposed to BPDE in vitro and to B[a]P in vivo. Environ Pollut 235:836-849 (2018). Mouse . Read more (PubMed: 29353801) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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