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Recombinant full length protein corresponding to Human Rap1 aa 1-184.
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQ CMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQI LRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKS KINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL
Our Abpromise guarantee covers the use of ab175329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 21 kDa.|
|IP||Use at 2 µg/mg of lysate.|
|ICC/IF||1/50 - 1/200.|
Immunofluorescence analysis of formalin-fixed permeabilized HeLa cells, labeling Rap1 (green, left panel) using ab175329 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).
Western blot analysis on immunoprecipitation pellet from mouse NIH 3T3 cells. The antigen-antibody complex was formed by incubating 750 µg of NIH 3T3 whole cell lysate with 2 µg of ab175329 overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose, washes extensively and eluted in sample buffer. 1) 25 µg of NIH 3T3 whole cell lysate, as a control, and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175329 at a 1/500 dilution. Chemiluminescent detection was perfomed.
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