Key features and details
- Rabbit polyclonal to RAP1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAP1 antibody
See all RAP1 primary antibodies
DescriptionRabbit polyclonal to RAP1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Tilapia, Orangutan
Synthetic peptide corresponding to Human RAP1 aa 475-525.
- HeLa whole cell lysate
Protein previously labeled as Rabex5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab103520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
Sequence similaritiesContains 1 A20-type zinc finger.
Contains 1 VPS9 domain.
Cellular localizationCytoplasm. Early endosome. Recycling endosome.
- Information by UniProt
- RAB guanine nucleotide exchange factor (GEF) 1 antibody
- Rab5 GDP/GTP exchange factor antibody
- Rab5ef antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RAP1 with ab103520 at 1/1000 (1µg/ml). Detection: DAB.
Detection of RAP1 by Western Blot of Immunprecipitate.
An antibody to RAP1 at 1µg/ml staining RAP1 in HeLa whole cell lysate immunoprecipitated using ab103520 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
ab103520 has not yet been referenced specifically in any publications.