Key features and details
- Rabbit polyclonal to RAP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RAP1 antibody
See all RAP1 primary antibodies
DescriptionRabbit polyclonal to RAP1
At least four isoforms of RAP1 are known to exist; ab113480 will recognize all four. ab113480 is predicted to not cross-react with other LRRFIP family members.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human RAP1 aa 1-19 (internal sequence).
- Rat brain tissue lysate
Protein previously labeled as Rabex5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab113480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 79 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionRab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
Sequence similaritiesContains 1 A20-type zinc finger.
Contains 1 VPS9 domain.
Cellular localizationCytoplasm. Early endosome. Recycling endosome.
- Information by UniProt
- RAB guanine nucleotide exchange factor (GEF) 1 antibody
- Rab5 GDP/GTP exchange factor antibody
- Rab5ef antibody
Lane 1 : Anti-RAP1 antibody (ab113480) at 1 µg/ml
Lane 2 : Anti-RAP1 antibody (ab113480) at 2 µg/ml
All lanes : Human liver tissue lysate
Predicted band size: 79 kDa
Immunohistochemical staining of RAP1 in mouse brain tissue with ab113480 at 20 ug/mL.
Anti-RAP1 antibody (ab113480) at 1 µg/ml + Rat brain tissue lysate at 15 µg
Predicted band size: 79 kDa
Immunohistochemical analysis of mouse brain tissue, staining RAP1 with ab113480 at 5 µg/ml.
ab113480 has been referenced in 1 publication.
- Kang L et al. RABEX-5 overexpression in gastric cancer is correlated with elevated MMP-9 level. Am J Transl Res 8:2365-74 (2016). PubMed: 27347344