Overview

  • Product name
  • Description
    Mouse polyclonal to RAP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human RAP1.

  • Positive control
    • Human liver tissue lysate and Rabex5 transfected 293T cell lysate.
  • General notes

    Protein previously labeled as Rabex5.

Properties

Applications

Our Abpromise guarantee covers the use of ab69437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
  • Sequence similarities
    Contains 1 A20-type zinc finger.
    Contains 1 VPS9 domain.
  • Post-translational
    modifications
    Monoubiquitinated.
  • Cellular localization
    Cytoplasm. Early endosome. Recycling endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • RAB guanine nucleotide exchange factor (GEF) 1 antibody
    • Rab5 GDP/GTP exchange factor antibody
    • Rab5ef antibody
    • Rabaptin 5 associated exchange factor for Rab5 antibody
    • Rabaptin-5-associated exchange factor for Rab5 antibody
    • Rabex 5 antibody
    • Rabex-5 antibody
    • RABGEF 1 antibody
    • Rabgef1 antibody
    • RABX 5 antibody
    • RABX5 antibody
    • RABX5_HUMAN antibody
    • RAP1 antibody
    • Rin 2 antibody
    • Rin2 antibody
    see all

Images

  • Anti-RAP1 antibody (ab69437) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-RAP1 antibody (ab69437) at 1/500 dilution

    Lane 1 : Rabex5 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

References

ab69437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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