Product nameAnti-RAP1A + RAP1B antibody [EP879Y]
See all RAP1A + RAP1B primary antibodies
DescriptionRabbit monoclonal [EP879Y] to RAP1A + RAP1B
Tested applicationsSuitable for: WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RAP1A + RAP1B (N terminal). The exact sequence is proprietary.
- Human brain lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|ICC||1/100 - 1/250.|
RelevanceRAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.
Cellular localizationCell Membrane; Attached to the membrane by a lipid anchor
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This product has been referenced in:
- Xu B et al. GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose. Brain Res Bull 142:163-175 (2018). Read more (PubMed: 30031816) »
- Todorovic V et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). Read more (PubMed: 25208567) »