Overview

  • Product name

    Anti-RAP1A + RAP1B antibody [EP879Y]
    See all RAP1A + RAP1B primary antibodies
  • Description

    Rabbit monoclonal [EP879Y] to RAP1A + RAP1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAP1A + RAP1B (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP879Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Relevance

      RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.
    • Cellular localization

      Cell Membrane; Attached to the membrane by a lipid anchor
    • Database links

    • Alternative names

      • C21KG antibody
      • G 22K antibody
      • GTP binding protein smg p21A antibody
      • GTP binding protein smg p21B antibody
      • K REV antibody
      • KREV 1 antibody
      • KREV antibody
      • KREV1 antibody
      • RAL 1B antibody
      • RAL1B antibody
      • RAP 1 antibody
      • RAP 1A antibody
      • RAP 1B antibody
      • RAP1 antibody
      • RAP1A member of RAS oncogene family antibody
      • RAP1B antibody
      • RAP1B member of RAS oncogene family antibody
      • Ras family small GTP binding protein RAP1B antibody
      • RAS related protein 1b antibody
      • Ras related protein Krev 1 antibody
      • Ras related protein Krev1 antibody
      • Ras related protein RAP 1A antibody
      • Ras related protein RAP 1B antibody
      • RAS related protein RAP1A antibody
      • Ras related protein RAP1B antibody
      • small GTP binding protein antibody
      • SMGP 21 antibody
      • SMGP21 antibody
      see all

    Images

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain

      Predicted band size: 21 kDa



      Secondary antibody - goat anti-rabbit HRP (ab6721)

    References

    This product has been referenced in:

    • Xu B  et al. GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose. Brain Res Bull 142:163-175 (2018). Read more (PubMed: 30031816) »
    • Todorovic V  et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). Read more (PubMed: 25208567) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEPG2)
    Specification
    HEPG2
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    20 µg
    Specification
    HEPG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Jan 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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