Recombinant Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14814] to RAP1A + RAP1B
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAP1A + RAP1B antibody [EPR14814]
See all RAP1A + RAP1B primary antibodies -
Description
Rabbit monoclonal [EPR14814] to RAP1A + RAP1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293, MOLT4, C6, Raw264.7, PC12, NIH 3T3 and A431 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14814 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 21 kDa.
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IP |
1/50 - 1/70.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 21 kDa. |
IP
1/50 - 1/70. |
Target
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Relevance
RAP1A: Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function. -
Cellular localization
Cell membrane; Lipid-anchor. Cytoplasm. Cytoplasm › perinuclear region. Cell junction By similarity. Early endosome By similarity. -
Database links
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: 179530 Human
see all -
Alternative names
- C21KG antibody
- G-22K antibody
- GTP-binding protein smg p21A antibody
see all
Images
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All lanes : Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
All lanes : Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunoprecipitation analysis of A431 cell lysate labeling RAP1A + RAP1B with ab187659 at 1/50 dilution. Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgGat 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab187659 has not yet been referenced specifically in any publications.