Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-RAP1A + RAP1B antibody [EPR14815(B)]
See all RAP1A + RAP1B primary antibodies
DescriptionRabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAP1A + RAP1B aa 100 to the C-terminus. The exact sequence is proprietary.
- HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
RelevanceRAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.
Cellular localizationCell Membrane; Attached to the membrane by a lipid anchor
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
ab181858 has not yet been referenced specifically in any publications.