Recombinant
RabMAb

Recombinant Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

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Overview

  • Product name

    Anti-RAP1A + RAP1B antibody [EPR14815(B)]
    See all RAP1A + RAP1B primary antibodies

  • Description

    Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAP1A + RAP1B aa 100 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/50.
WB 1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Relevance

    RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.

  • Cellular localization

    Cell Membrane; Attached to the membrane by a lipid anchor

  • Database links

    see all

  • Alternative names

    • C21KG antibody
    • G 22K antibody
    • GTP binding protein smg p21A antibody
    • GTP binding protein smg p21B antibody
    • K REV antibody
    • KREV 1 antibody
    • KREV antibody
    • KREV1 antibody
    • RAL 1B antibody
    • RAL1B antibody
    • RAP 1 antibody
    • RAP 1A antibody
    • RAP 1B antibody
    • RAP1 antibody
    • RAP1A member of RAS oncogene family antibody
    • RAP1B antibody
    • RAP1B member of RAS oncogene family antibody
    • Ras family small GTP binding protein RAP1B antibody
    • RAS related protein 1b antibody
    • Ras related protein Krev 1 antibody
    • Ras related protein Krev1 antibody
    • Ras related protein RAP 1A antibody
    • Ras related protein RAP 1B antibody
    • RAS related protein RAP1A antibody
    • Ras related protein RAP1B antibody
    • small GTP binding protein antibody
    • SMGP 21 antibody
    • SMGP21 antibody
    see all

Images

  • Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Flow Cytometry - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Flow Cytometry - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab181858 has not yet been referenced specifically in any publications.

Publishing research using ab181858? Please let us know so that we can cite the reference in this datasheet.

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