Recombinant Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
![Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)](https://www.abcam.com/ps/products/181/ab181858/Images/ab181858-215327-anti-rap1a-rap1b-antibody-epr14815b-western-blot.jpg "Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAP1A + RAP1B antibody [EPR14815(B)]
See all RAP1A + RAP1B primary antibodies -
Description
Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14815(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/50.
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| WB |
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP
1/50. |
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WB
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Relevance
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
Cellular localization
Cell Membrane; Attached to the membrane by a lipid anchor -
Database links
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
see all -
Alternative names
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
see all
Images
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Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)](https://www.abcam.com/ps/products/181/ab181858/Images/ab181858-215326-anti-rap1a-rap1b-antibody-epr14815b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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![Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)](https://www.abcam.com/ps/products/181/ab181858/Images/ab181858-215325-anti-rap1a-rap1b-antibody-epr14815b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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![Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)](https://www.abcam.com/ps/products/181/ab181858/Images/ab181858-4-benefits-of-recombinant-antibodies.png)
Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab181858 has not yet been referenced specifically in any publications.
