Anti-RAP1A antibody (ab96223)
Key features and details
- Rabbit polyclonal to RAP1A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP1A antibody
See all RAP1A primary antibodies -
Description
Rabbit polyclonal to RAP1A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Zebrafish -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-154 of Human RAP1A (NP_001010935).
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Positive control
- MOLT4 whole cell lysate; 293T, A431, H1299, HeLa, HepG2 and Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
Target
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Function
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5906 Human
- Entrez Gene: 295347 Rat
- Entrez Gene: 415242 Zebrafish
- Omim: 179520 Human
- SwissProt: P62834 Human
- SwissProt: P62836 Rat
- Unigene: 190334 Human
- Unigene: 106321 Rat
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Alternative names
- C21KG antibody
- G 22K antibody
- G-22K antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab96223 has been referenced in 4 publications.
- Burr SD et al. Rap1a Activity Elevated the Impact of Endogenous AGEs in Diabetic Collagen to Stimulate Increased Myofibroblast Transition and Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 35562872
- Ding X et al. EIF3C Promotes Lung Cancer Tumorigenesis by Regulating the APP/HSPA1A/LMNB1 Axis. Dis Markers 2022:9464094 (2022). PubMed: 36157221
- Burr SD & Stewart JA Rap1a Overlaps the AGE/RAGE Signaling Cascade to Alter Expression of a-SMA, p-NF-?B, and p-PKC-? in Cardiac Fibroblasts Isolated from Type 2 Diabetic Mice. Cells 10:N/A (2021). PubMed: 33806572
- Chen JH et al. Long noncoding RNA CRNDE promotes proliferation, migration and invasion in prostate cancer through miR-101/Rap1A. Neoplasma 67:584-594 (2020). PubMed: 32182086