Overview

  • Product name

    Anti-RAP1A + RAP1B antibody
    See all RAP1A + RAP1B primary antibodies
  • Description

    Rabbit polyclonal to RAP1A + RAP1B
  • Host species

    Rabbit
  • Specificity

    ab25980 recognises Rap1.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Human Rap synthetic peptide Rap1a (a.a. 31-48), identical to Rap1b except that Rap1b has an alanine at residue 48, and the peptide conjugated to KLH.

  • Positive control

    • Rat Brain Tissue Extract Mouse Brain Tissue Extract

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa.

Target

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Dictyostelium discoideum Cell lysate - whole cell (Dictyostelium discoideum, RAW 264.7 cells)
Loading amount
1000 cells
Specification
Dictyostelium discoideum, RAW 264.7 cells
Gel Running Conditions
Reduced Denaturing (12 % gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

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Verified customer

Submitted Jul 06 2010

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