Western blot abreview for Anti-RAP1A + RAP1B antibody

Average
Abreviews
Application
Western blot
Sample
Dictyostelium discoideum Cell lysate - whole cell (Dictyostelium discoideum, RAW 264.7 cells)
Loading amount
1000 cells
Specification
Dictyostelium discoideum, RAW 264.7 cells
Gel Running Conditions
Reduced Denaturing (12 % gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 24 kDa Non-specific: 20 kDa
Positive control
Lane 5: RAW 264.7 mammalian cells showed a band of around 24 kDa. However, Dictyostelium cells showed a non-specific band of around 20 kDa, instead of 24 kDa.

Additional data

Additional Notes
Discount code: 51716
Expiration date: 12-Aug-10

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Submitted Jul 06 2010

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