Key features and details
- Rabbit polyclonal to RAP1B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RAP1B antibody
See all RAP1B primary antibodies
DescriptionRabbit polyclonal to RAP1B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length protein corresponding to Human RAP1B. (With tag; BC000176).
Database link: P61224
- HeLa, HepG2 and Human cervical cancer tissue lsyates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 21 kDa.|
FunctionGTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
Cellular localizationCell membrane. Cytoplasm > cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
- Information by UniProt
- GTP-binding protein smg p21B antibody
- K-REV antibody
- OK/SW-cl.11 antibody
All lanes : Anti-RAP1B antibody (ab182606) at 1/700 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human cervical cancer tissue lysate
Lysates/proteins at 24 µg per lane.
All lanes : Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Predicted band size: 21 kDa
Exposure time: 1 minute
Gel: 10% SDS-PAGE
ab182606 has not yet been referenced specifically in any publications.