Overview

  • Product name

  • Description

    Rabbit polyclonal to RAP1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human RAP1B. (With tag; BC000176).
    Database link: P61224

  • Positive control

    • HeLa, HepG2 and Human cervical cancer tissue lsyates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • Function

    GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.
  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP-binding protein smg p21B antibody
    • K-REV antibody
    • OK/SW-cl.11 antibody
    • RAL1B antibody
    • rap1b antibody
    • RAP1B member of RAS oncogene family antibody
    • RAP1B_HUMAN antibody
    • Ras family small GTP binding protein RAP1B antibody
    • RAS related protein RAP1B antibody
    • Ras-related protein Rap-1b antibody
    • Small GTP binding protein antibody
    see all

Images

  • All lanes : Anti-RAP1B antibody (ab182606) at 1/700 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human cervical cancer tissue lysate

    Lysates/proteins at 24 µg per lane.

    Secondary
    All lanes : Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution

    Predicted band size: 21 kDa


    Exposure time: 1 minute


    Gel: 10% SDS-PAGE

References

ab182606 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab182606.
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