Anti-RAP1B antibody (ab182606)
Key features and details
- Rabbit polyclonal to RAP1B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP1B antibody
See all RAP1B primary antibodies -
Description
Rabbit polyclonal to RAP1B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant full length protein corresponding to Human RAP1B. (With tag; BC000176).
Database link: P61224 -
Positive control
- HeLa, HepG2 and Human cervical cancer tissue lsyates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Note: PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
Target
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Function
GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction. - Information by UniProt
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Database links
- Entrez Gene: 5908 Human
- Entrez Gene: 171337 Rat
- Omim: 179530 Human
- SwissProt: P61224 Human
- SwissProt: Q62636 Rat
- Unigene: 369920 Human
- Unigene: 95071 Rat
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Alternative names
- GTP-binding protein smg p21B antibody
- K-REV antibody
- OK/SW-cl.11 antibody
see all
Images
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All lanes : Anti-RAP1B antibody (ab182606) at 1/700 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human cervical cancer tissue lysate
Lysates/proteins at 24 µg per lane.
Secondary
All lanes : Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Predicted band size: 21 kDa
Exposure time: 1 minuteGel: 10% SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab182606 has been referenced in 1 publication.
- Wang L et al. LINC00665 Facilitates the Malignant Processes of Osteosarcoma by Increasing the RAP1B Expression via Sponging miR-708 and miR-142-5p. Anal Cell Pathol (Amst) 2021:5525711 (2021). PubMed: 34306997