Product nameAnti-RAP55B antibody
DescriptionRabbit polyclonal to RAP55B
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human testis tissue. ICC/IF: SiHa cells.
Previously labelled as FAM61B.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/100 - 1/250. Predicted molecular weight: 42 kDa.|
RelevanceRAP55B belongs to the LSM14 family and may play a role in control of mRNA translation.
- Protein FAM61B antibody
- Putative uncharacterized protein C20orf40 antibody
- bA11M20.3 antibody
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human testis tissue sections labeling RAP55B with ab221041 at a 1/50 dilution.
All lanes : Anti-RAP55B antibody (ab221041) at 1/100 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : RAP55B over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Predicted band size: 42 kDa
Immunofluorescent analysis of PFA fixed, triton X-100 SiHa cells labeling RAP55B with ab221041 at 4 µg/mL (green).
ab221041 has not yet been referenced specifically in any publications.