Key features and details
- Rabbit polyclonal to RAP80
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAP80 antibody
See all RAP80 primary antibodies
DescriptionRabbit polyclonal to RAP80
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
- IHC-P: Human lymph node, colon, testis and cerebral cortex tissue. WB: HDML-2 cell lysate ICC/IF: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
Our Abpromise guarantee covers the use of ab244465 in the following tested applications.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionUbiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Tissue specificityExpressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Sequence similaritiesBelongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
DomainThe UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
Phosphorylated upon DNA damage by ATM or ATR.
Cellular localizationNucleus. Localizes at sites of DNA damage at double-strand breaks.
- Information by UniProt
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
Anti-RAP80 antibody (ab244465) at 0.4 µg/ml + HDML-2 cell lysate
Paraffin-embedded human cerebral cortex tissue stained for RAP80 using ab244465 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RAP80 (green) using ab244465 at 4 μg/ml in ICC/IF.
Paraffin-embedded human colon tissue stained for RAP80 using ab244465 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for RAP80 using ab244465 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for RAP80 using ab244465 at 1/50 dilution in immunohistochemical analysis.
ab244465 has not yet been referenced specifically in any publications.