Anti-RAP80 antibody (ab70822)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RAP80 antibody
See all RAP80 primary antibodies -
Description
Rabbit polyclonal to RAP80 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | ||
| WB |
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. -
Tissue specificity
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. -
Sequence similarities
Belongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Domain
The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. -
Post-translational
modificationsSumoylated.
Phosphorylated upon DNA damage by ATM or ATR. -
Cellular localization
Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 462299 Chimpanzee
- Entrez Gene: 51720 Human
- SwissProt: Q96RL1 Human
- Unigene: 232721 Human
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Alternative names
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
see all
Images
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Western blot - Anti-RAP80 antibody (ab70822)All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-RAP80 antibody (ab70822)Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
References
This product has been referenced in:
- Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). Read more (PubMed: 25939603) »
