Anti-RAP80 antibody (ab70822)
Key features and details
- Rabbit polyclonal to RAP80
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP80 antibody
See all RAP80 primary antibodies -
Description
Rabbit polyclonal to RAP80 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Chimpanzee
Gorilla
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
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| Notes |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa). |
Target
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Function
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. -
Tissue specificity
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. -
Sequence similarities
Belongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats. -
Domain
The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. -
Post-translational
modificationsSumoylated.
Phosphorylated upon DNA damage by ATM or ATR. -
Cellular localization
Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 462299 Chimpanzee
- Entrez Gene: 51720 Human
- SwissProt: Q96RL1 Human
- Unigene: 232721 Human
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Alternative names
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
see all
Images
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Western blot - Anti-RAP80 antibody (ab70822)All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-RAP80 antibody (ab70822)Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70822 has been referenced in 1 publication.
- Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). PubMed: 25939603