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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-RAP80 antibody (ab70822)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-RAP80 antibody (ab70822)
  • Immunoprecipitation - Anti-RAP80 antibody (ab70822)

Key features and details

  • Rabbit polyclonal to RAP80
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RAP80 antibody
    See all RAP80 primary antibodies
  • Description

    Rabbit polyclonal to RAP80
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin Binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Chimpanzee
Gorilla
Application Abreviews Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
Notes
IP
Use at 2-5 µg/mg of lysate.
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).

Target

  • Function

    Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
  • Tissue specificity

    Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
  • Sequence similarities

    Belongs to the RAP80 family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Domain

    The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
    The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
  • Post-translational
    modifications

    Sumoylated.
    Phosphorylated upon DNA damage by ATM or ATR.
  • Cellular localization

    Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Target information above from: UniProt accession Q96RL1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 462299 Chimpanzee
    • Entrez Gene: 51720 Human
    • SwissProt: Q96RL1 Human
    • Unigene: 232721 Human
    • Alternative names

      • BRCA1-A complex subunit RAP80 antibody
      • Nuclear zinc finger protein RAP80 antibody
      • OTTHUMP00000161441 antibody
      • OTTHUMP00000223372 antibody
      • OTTHUMP00000223374 antibody
      • RAP80 antibody
      • Receptor associated protein 80 antibody
      • Receptor-associated protein 80 antibody
      • Retinoid X receptor interacting protein 110 antibody
      • Retinoid x receptor interacting protein antibody
      • Retinoid X receptor-interacting protein 110 antibody
      • RIP110 antibody
      • Rxrip110 antibody
      • Ubiquitin interaction motif containing 1 antibody
      • Ubiquitin interaction motif containing protein 1 antibody
      • Ubiquitin interaction motif-containing protein 1 antibody
      • UIMC1 antibody
      • UIMC1_HUMAN antibody
      • X2HRIP110 antibody
      see all

    Images

    • Western blot - Anti-RAP80 antibody (ab70822)
      Western blot - Anti-RAP80 antibody (ab70822)
      All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate at 15 µg
      Lane 2 : HeLa whole cell lysate at 5 µg
      Lane 3 : 293T whole cell lysate at 50 µg

      Predicted band size: 80 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?
      Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - Anti-RAP80 antibody (ab70822)
      Immunoprecipitation - Anti-RAP80 antibody (ab70822)
      Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab70822? Please let us know so that we can cite the reference in this datasheet.

    ab70822 has been referenced in 1 publication.

    • Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). PubMed: 25939603

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-RAP80 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF7 cells)
    Specification
    MCF7 cells
    Fixative
    Methanol
    Permeabilization
    Yes - Acetone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 21 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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