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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response DNA Damage Recognition
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Anti-RAP80 antibody (ab70822)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Abpromise

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Western blot - Anti-RAP80 antibody (ab70822)
  • Immunoprecipitation - Anti-RAP80 antibody (ab70822)

Key features and details

  • Rabbit polyclonal to RAP80
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RAP80 antibody
    See all RAP80 primary antibodies
  • Description

    Rabbit polyclonal to RAP80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin Binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab70822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
    • Tissue specificity

      Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
    • Sequence similarities

      Belongs to the RAP80 family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • Domain

      The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
      The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
    • Post-translational
      modifications

      Sumoylated.
      Phosphorylated upon DNA damage by ATM or ATR.
    • Cellular localization

      Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Target information above from: UniProt accession Q96RL1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 462299 Chimpanzee
      • Entrez Gene: 51720 Human
      • SwissProt: Q96RL1 Human
      • Unigene: 232721 Human
      • Alternative names

        • BRCA1-A complex subunit RAP80 antibody
        • Nuclear zinc finger protein RAP80 antibody
        • OTTHUMP00000161441 antibody
        • OTTHUMP00000223372 antibody
        • OTTHUMP00000223374 antibody
        • RAP80 antibody
        • Receptor associated protein 80 antibody
        • Receptor-associated protein 80 antibody
        • Retinoid X receptor interacting protein 110 antibody
        • Retinoid x receptor interacting protein antibody
        • Retinoid X receptor-interacting protein 110 antibody
        • RIP110 antibody
        • Rxrip110 antibody
        • Ubiquitin interaction motif containing 1 antibody
        • Ubiquitin interaction motif containing protein 1 antibody
        • Ubiquitin interaction motif-containing protein 1 antibody
        • UIMC1 antibody
        • UIMC1_HUMAN antibody
        • X2HRIP110 antibody
        see all

      Images

      • Western blot - Anti-RAP80 antibody (ab70822)
        Western blot - Anti-RAP80 antibody (ab70822)
        All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml

        Lane 1 : HeLa whole cell lysate at 15 µg
        Lane 2 : HeLa whole cell lysate at 5 µg
        Lane 3 : 293T whole cell lysate at 50 µg

        Predicted band size: 80 kDa
        Observed band size: 100 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

      • Immunoprecipitation - Anti-RAP80 antibody (ab70822)
        Immunoprecipitation - Anti-RAP80 antibody (ab70822)
        Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab70822? Please let us know so that we can cite the reference in this datasheet.

      ab70822 has been referenced in 1 publication.

      • Goldstein M & Kastan MB Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res 75:2699-707 (2015). PubMed: 25939603

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Filter by Application

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-RAP80 antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (MCF7 cells)
      Specification
      MCF7 cells
      Fixative
      Methanol
      Permeabilization
      Yes - Acetone
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 21 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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