Product nameAnti-RAP80 antibody [EPR5315]
See all RAP80 primary antibodies
DescriptionRabbit monoclonal [EPR5315] to RAP80
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human RAP80 aa 1-100. The exact sequence is proprietary.
- HeLa, Human ovary cancer, 293T and HT-1080 cell lysates; Human testis tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
Our Abpromise guarantee covers the use of ab124763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
|ICC/IF||1/50 - 1/100.|
FunctionUbiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Tissue specificityExpressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Sequence similaritiesBelongs to the RAP80 family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
DomainThe UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
Phosphorylated upon DNA damage by ATM or ATR.
Cellular localizationNucleus. Localizes at sites of DNA damage at double-strand breaks.
- Information by UniProt
- BRCA1-A complex subunit RAP80 antibody
- Nuclear zinc finger protein RAP80 antibody
- OTTHUMP00000161441 antibody
All lanes : Anti-RAP80 antibody [EPR5315] (ab124763) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : 293T cell lysate
Lane 4 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
ab124763, at 1/250, staining RAP80 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Liu P et al. K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair. Sci Signal 11:N/A (2018). Read more (PubMed: 29871913) »
- Renaud E et al. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res 44:648-56 (2016). WB ; Human . Read more (PubMed: 26446986) »