Overview

  • Product name

    Anti-RAPGEF5 antibody [EPR6882]
    See all RAPGEF5 primary antibodies
  • Description

    Rabbit monoclonal [EPR6882] to RAPGEF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAPGEF5 (N terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, and K562 cell lysates; Human testis and brain tissues; 293 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.
    • Tissue specificity

      Widely expressed with highest levels in brain.
    • Sequence similarities

      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 Ras-GEF domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • GFR antibody
      • Guanine nucleotide exchange factor for Rap1 antibody
      • KIAA0277 antibody
      • M Ras regulated Rap GEF antibody
      • M-Ras-regulated GEF antibody
      • M-Ras-regulated Rap GEF antibody
      • MR GEF antibody
      • MR-GEF antibody
      • MRas-regulated guanine nucleotide exchange factor antibody
      • MRGEF antibody
      • Rap guanine nucleotide exchange factor (GEF) 5 antibody
      • Rap guanine nucleotide exchange factor 5 antibody
      • Rapgef5 antibody
      • Related to Epac antibody
      • Repac antibody
      • RPGF5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RAPGEF5 antibody [EPR6882] (ab129008) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 68 kDa

    • ab129008, at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human testis tissue by Immunohistochemistry.
    • ab129008, at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab129008, at 1/100 dilution, staining RAPGEF5 in 293 cells by Immunofluorescence
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Griffin JN  et al. RAPGEF5 Regulates Nuclear Translocation of ß-Catenin. Dev Cell 44:248-260.e4 (2018). Read more (PubMed: 29290587) »
    • Chen W  et al. Identification of chromosomal copy number variations and novel candidate loci in hereditary nonpolyposis colorectal cancer with mismatch repair proficiency. Genomics 102:27-34 (2013). IHC-P ; Human . Read more (PubMed: 23434627) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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