Product nameAnti-RAPGEF5 antibody [EPR6882]
See all RAPGEF5 primary antibodies
DescriptionRabbit monoclonal [EPR6882] to RAPGEF5
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RAPGEF5 (N terminal). The exact sequence is proprietary.
- 293T, HeLa, and K562 cell lysates; Human testis and brain tissues; 293 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionGuanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.
Tissue specificityWidely expressed with highest levels in brain.
Sequence similaritiesContains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.
- Information by UniProt
- GFR antibody
- Guanine nucleotide exchange factor for Rap1 antibody
- KIAA0277 antibody
All lanes : Anti-RAPGEF5 antibody [EPR6882] (ab129008) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 68 kDa
ab129008, at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human testis tissue by Immunohistochemistry.
ab129008, at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human brain tissue by Immunohistochemistry.
ab129008, at 1/100 dilution, staining RAPGEF5 in 293 cells by Immunofluorescence
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Griffin JN et al. RAPGEF5 Regulates Nuclear Translocation of ß-Catenin. Dev Cell 44:248-260.e4 (2018). Read more (PubMed: 29290587) »
- Chen W et al. Identification of chromosomal copy number variations and novel candidate loci in hereditary nonpolyposis colorectal cancer with mismatch repair proficiency. Genomics 102:27-34 (2013). IHC-P ; Human . Read more (PubMed: 23434627) »