Key features and details
- Rabbit polyclonal to RAPH1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAPH1 antibody
DescriptionRabbit polyclonal to RAPH1
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to C terminal amino acids 1105-1248 of Human RAPH1.
- Human duodenum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6|
FunctionMediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
Tissue specificityIsoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.
Sequence similaritiesBelongs to the MRL family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Cellular localizationCell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
- Information by UniProt
- 9430025M21Rik antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane and cytoplasm. Recommended concentration of ab121619 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121619 at 1/75 staining RALPH1 in Paraffin Embedded Human duodenum tissue. Note strong cytoplasmic positivity in glandular cells.
ab121619 has not yet been referenced specifically in any publications.