Recombinant Anti-Rapsyn antibody [EPR9759] (ab156002)
![Western blot - Anti-Rapsyn antibody [EPR9759] (ab156002)](https://www.abcam.com/ps/products/156/ab156002/Images/ab156002-163337-anti-rapsyn-antibody-epr9759-western-blot.jpg "Western blot - Anti-Rapsyn antibody [EPR9759] (ab156002)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9759] to Rapsyn
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Rapsyn antibody [EPR9759]
See all Rapsyn primary antibodies -
Description
Rabbit monoclonal [EPR9759] to Rapsyn -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- L6 and C2C12 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9759 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 46 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 46 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Thought to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. -
Involvement in disease
Defects in RAPSN are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (ACHRDCMS) [MIM:608931]. ACHRDCMS is a post-synaptic congenital myasthenic syndrome. Congenital myasthenic syndromes (CMS) are inherited disorders of neuromuscular transmission that stem from mutations in presynaptic, synaptic, or postsynaptic proteins. Postsynaptic disorders result from mutations in proteins forming the subunits of the muscle acetylcholine receptor (AChR). The kinetic abnormalities of AChR result in either prolonged ion channel activations that underlie 'slow-channel myasthenic syndromes' (SCCMS) or abbreviated channel activations that underlie the abnormally rapid decay of endplate currents in 'fast-channel syndromes' (FCCMS). ACHRDCMS is the third disorder associated with postsynaptic CMS which could result from mutations in the proteins forming the muscle AChR. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance.
Defects in RAPSN are the cause of fetal akinesia deformation sequence (FADS) [MIM:208150]; also known as Pena-Shokeir syndrome type 1 or fetal akinesia sequence or arthrogryposis multiplex congenita with pulmonary hypoplasia. FADS is a rare condition characterized by decreased intrauterine fetal movement, congenital limb contractures, pulmonary hypoplasia, polyhydramnios and craniofacial abnormalities. -
Sequence similarities
Belongs to the RAPsyn family.
Contains 1 RING-type zinc finger.
Contains 7 TPR repeats. -
Domain
A cysteine-rich region homologous to part of the regulatory domain of protein kinase C may be important in interactions of this protein with the lipid bilayer. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic surface of postsynaptic membranes. - Information by UniProt
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Database links
- Entrez Gene: 5913 Human
- Entrez Gene: 19400 Mouse
- Entrez Gene: 362161 Rat
- Omim: 601592 Human
- SwissProt: Q13702 Human
- SwissProt: P12672 Mouse
- Unigene: 81218 Human
- Unigene: 1272 Mouse
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Alternative names
- 43 kD receptor associated protein of the synapse antibody
- 43 kDa postsynaptic protein antibody
- 43 kDa receptor-associated protein of the synapse antibody
see all
Images
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Western blot - Anti-Rapsyn antibody [EPR9759] (ab156002)All lanes : Anti-Rapsyn antibody [EPR9759] (ab156002) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
![Anti-Rapsyn antibody [EPR9759] (ab156002)](https://www.abcam.com/ps/products/156/ab156002/Images/ab156002-2-benefits-of-recombinant-antibodies.png)
Anti-Rapsyn antibody [EPR9759] (ab156002)
Datasheets and documents
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Datasheet download
References (6)
ab156002 has been referenced in 6 publications.
- Pezinski M et al. An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery. Sci Rep 10:4524 (2020). PubMed: 32161296
- Li B et al. Stability of motor endplates is greater in the biceps than in the interossei in a rat model of obstetric brachial plexus palsy. Neural Regen Res 15:1678-1685 (2020). PubMed: 32209772
- Oury J et al. MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses. J Cell Biol 218:1686-1705 (2019). PubMed: 30842214
- Koppel N et al. Vezatin is required for the maturation of the neuromuscular synapse. Mol Biol Cell 30:2571-2583 (2019). PubMed: 31411944
- Kelly NA et al. Effects of aging and Parkinson's disease on motor unit remodeling: influence of resistance exercise training. J Appl Physiol (1985) 124:888-898 (2018). PubMed: 29357501
- Bernadzki KM et al. Liprin-a-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery. Sci Rep 7:9116 (2017). PubMed: 28831123
