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Anti-Raptor antibody (ab125351)

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Western blot - Anti-Raptor antibody (ab125351)

    Key features and details

    • Rabbit polyclonal to Raptor
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Raptor protein (ab112419)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Raptor antibody
      See all Raptor primary antibodies
    • Description

      Rabbit polyclonal to Raptor
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla
    • Immunogen

      Synthetic peptide corresponding to Human Raptor aa 500-600 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab155813)

    • Positive control

      • This antibody gave a positive signal in the following human whole cell lysates: HepG2; HeLa; HEK293; PANC1; A431.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Other
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cell Cycle Inhibitors
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Raptor protein (ab112419)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab125351 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).
    Notes
    WB
    1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).

    Target

    • Function

      Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
    • Tissue specificity

      Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
    • Sequence similarities

      Belongs to the WD repeat RAPTOR family.
      Contains 7 WD repeats.
    • Cellular localization

      Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
    • Target information above from: UniProt accession Q8N122 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57521 Human
      • Omim: 607130 Human
      • SwissProt: Q8N122 Human
      • Unigene: 133044 Human
      • Alternative names

        • KIAA1303 antibody
        • KOG1 antibody
        • Mip1 antibody
        • P150 target of rapamycin (TOR) scaffold protein antibody
        • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
        • P150 target of rapamycin (TOR)-scaffold protein antibody
        • Raptor antibody
        • Regulatory associated protein of mTOR antibody
        • Regulatory associated protein of MTOR complex 1 antibody
        • Regulatory-associated protein of mTOR antibody
        • RPTOR antibody
        • RPTOR_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Raptor antibody (ab125351)
        Western blot - Anti-Raptor antibody (ab125351)
        All lanes : Anti-Raptor antibody (ab125351) at 1/250 dilution

        Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
        Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 4 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
        Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 149 kDa
        Observed band size: 151 kDa why is the actual band size different from the predicted?
        Additional bands at: 60 kDa, 72 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 3 minutes


        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125351 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab125351? Please let us know so that we can cite the reference in this datasheet.

      ab125351 has not yet been referenced specifically in any publications.

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