Product nameAnti-Raptor antibody
See all Raptor primary antibodies
DescriptionMouse polyclonal to Raptor
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Full length protein corresponding to Human Raptor aa 1-379.
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWR MKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALE TIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHY NGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAG LIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEATE LLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPG RLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNF LLAERIMRSYNCTPVSSPRLPPTYMHAMW
Database link: AAH64515.1
- Raptor transfected 293T cell lysate; HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionInvolved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Tissue specificityHighly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Sequence similaritiesBelongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
- Information by UniProt
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
All lanes : Anti-Raptor antibody (ab169506) at 1 µg/ml
Lane 1 : Raptor transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Immunofluorescent analysis of HeLa cells labeling Raptor with ab169506 at 10 µg/ml.
This product has been referenced in:
- Poulopoulos A et al. Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex. Nature 565:356-360 (2019). Read more (PubMed: 30626971) »
- Bostner J et al. Raptor localization predicts prognosis and tamoxifen response in estrogen receptor-positive breast cancer. Breast Cancer Res Treat 168:17-27 (2018). Read more (PubMed: 29128895) »