Recombinant
RabMAb

Recombinant Anti-Raptor antibody [EP539Y] - BSA and Azide free (ab239827)

Overview

  • Product name

    Anti-Raptor antibody [EP539Y] - BSA and Azide free
    See all Raptor primary antibodies
  • Description

    Rabbit monoclonal [EP539Y] to Raptor - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Raptor aa 1300-1400 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab239827 is the carrier-free version of ab40768. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239827 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 149 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
    • Tissue specificity

      Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
    • Sequence similarities

      Belongs to the WD repeat RAPTOR family.
      Contains 7 WD repeats.
    • Cellular localization

      Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA1303 antibody
      • KOG1 antibody
      • Mip1 antibody
      • P150 target of rapamycin (TOR) scaffold protein antibody
      • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
      • P150 target of rapamycin (TOR)-scaffold protein antibody
      • Raptor antibody
      • Regulatory associated protein of mTOR antibody
      • Regulatory associated protein of MTOR complex 1 antibody
      • Regulatory-associated protein of mTOR antibody
      • RPTOR antibody
      • RPTOR_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40768).

    • Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40768).

    References

    ab239827 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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