Anti-Raptor antibody - N-terminal (ab226824)
Key features and details
- Rabbit polyclonal to Raptor - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Raptor antibody - N-terminal
See all Raptor primary antibodies -
Description
Rabbit polyclonal to Raptor - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide within Human Raptor (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q8N122 -
Positive control
- WB: HEK-293T, A431 and HeLa whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 149 kDa.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/5000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 149 kDa. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/5000. |
Target
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Function
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
Tissue specificity
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
Sequence similarities
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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Database links
- Entrez Gene: 57521 Human
- Entrez Gene: 287871 Rat
- Omim: 607130 Human
- SwissProt: Q8N122 Human
- Unigene: 133044 Human
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Alternative names
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
Images
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All lanes : Anti-Raptor antibody - N-terminal (ab226824) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 149 kDa5% SDS-PAGE gel.
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Paraffn-embedded rat intestine tissue stained for Raptor using ab226824 at 1/1500 dilution in immunohistochemical analysis.
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Raptor was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract using ab226824 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab226824 at 1/500 dilution.
Lane 1: HEK-293T whole cell extract.
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab226824 IP in HEK-293T whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226824 has been referenced in 1 publication.
- Zhu Y et al. Cardamonin inhibits cell proliferation by caspase-mediated cleavage of Raptor. Naunyn Schmiedebergs Arch Pharmacol 394:809-817 (2021). PubMed: 33043385