Overview

  • Product name

    Anti-Raptor antibody - N-terminal
    See all Raptor primary antibodies
  • Description

    Rabbit polyclonal to Raptor - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human Raptor (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q8N122

  • Positive control

    • WB: HEK-293T, A431 and HeLa whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 149 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/5000.

Target

  • Function

    Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
  • Tissue specificity

    Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
  • Sequence similarities

    Belongs to the WD repeat RAPTOR family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1303 antibody
    • KOG1 antibody
    • Mip1 antibody
    • P150 target of rapamycin (TOR) scaffold protein antibody
    • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
    • P150 target of rapamycin (TOR)-scaffold protein antibody
    • Raptor antibody
    • Regulatory associated protein of mTOR antibody
    • Regulatory associated protein of MTOR complex 1 antibody
    • Regulatory-associated protein of mTOR antibody
    • RPTOR antibody
    • RPTOR_HUMAN antibody
    see all

Images

  • All lanes : Anti-Raptor antibody - N-terminal (ab226824) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 149 kDa



    5% SDS-PAGE gel.

  • Paraffn-embedded rat intestine tissue stained for Raptor using ab226824 at 1/1500 dilution in immunohistochemical analysis.

  • Raptor was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract using ab226824 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab226824 at 1/500 dilution.

    Lane 1: HEK-293T whole cell extract.

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab226824 IP in HEK-293T whole cell extract.

References

ab226824 has not yet been referenced specifically in any publications.

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