Key features and details
- Rabbit polyclonal to Raptor - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Raptor antibody - N-terminal
See all Raptor primary antibodies
DescriptionRabbit polyclonal to Raptor - N-terminal
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Cow
Synthetic peptide within Human Raptor (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q8N122
- WB: HEK-293T, A431 and HeLa whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 149 kDa.|
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/5000.|
FunctionInvolved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Tissue specificityHighly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Sequence similaritiesBelongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
- Information by UniProt
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
All lanes : Anti-Raptor antibody - N-terminal (ab226824) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 149 kDa
5% SDS-PAGE gel.
Paraffn-embedded rat intestine tissue stained for Raptor using ab226824 at 1/1500 dilution in immunohistochemical analysis.
Raptor was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract using ab226824 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab226824 at 1/500 dilution.
Lane 1: HEK-293T whole cell extract.
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab226824 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226824 has not yet been referenced specifically in any publications.