Overview

  • Product name

  • Description

    Rabbit polyclonal to RARRES1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to RARRES1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87448)

  • Positive control

    • Recombinant Human RARRES1 protein (ab114592) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human Liver Tissue; Human Kidney Tissue; HepG2 Whole Cell; HEK293 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab76530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).

Target

  • Sequence similarities

    Belongs to the protease inhibitor I47 (latexin) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5430417P09Rik antibody
    • AI662122 antibody
    • RAR-responsive protein TIG1 antibody
    • RARRES1 antibody
    • Retinoic acid receptor responder (tazarotene induced) 1 antibody
    • Retinoic acid receptor responder protein 1 antibody
    • Tazarotene induced gene 1 protein antibody
    • Tazarotene-induced gene 1 protein antibody
    • TIG1 antibody
    • TIG1_HUMAN antibody
    see all

Images

  • All lanes : Anti-RARRES1 antibody (ab76530) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 62 kDa, 80 kDa. We are unsure as to the identity of these extra bands.



    RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Pang B  et al. Landscape of tumor suppressor long noncoding RNAs in breast cancer. J Exp Clin Cancer Res 38:79 (2019). Read more (PubMed: 30764831) »
  • Chen HL  et al. Secretome survey of human plexiform neurofibroma derived Schwann Cells reveals a secreted form of the RARRES1 protein. Int J Mol Sci 13:9380-99 (2012). Read more (PubMed: 22942771) »
See all 2 Publications for this product

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