Anti-RARRES1 antibody (ab87115)
Key features and details
- Rabbit polyclonal to RARRES1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RARRES1 antibody
See all RARRES1 primary antibodies -
Description
Rabbit polyclonal to RARRES1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human RARRES1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87814) -
Positive control
- Recombinant Human RARRES1 protein (ab114592) can be used as a positive control in WB. This antibody gave a positive signal in the following Whole cell lysates: HepG2; HEK293.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). |
Target
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Sequence similarities
Belongs to the protease inhibitor I47 (latexin) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5918 Human
- Omim: 605090 Human
- SwissProt: P49788 Human
- Unigene: 131269 Human
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Alternative names
- 5430417P09Rik antibody
- AI662122 antibody
- RAR-responsive protein TIG1 antibody
see all
Images
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Western blot - Anti-RARRES1 antibody (ab87115)All lanes : Anti-RARRES1 antibody (ab87115) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 40 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to RARRES1.
Datasheets and documents
References (0)
ab87115 has not yet been referenced specifically in any publications.
