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    rarres1-antibody-ab87115.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Tumor Suppressors
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Anti-RARRES1 antibody (ab87115)

  • Datasheet
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Western blot - Anti-RARRES1 antibody (ab87115)

    Key features and details

    • Rabbit polyclonal to RARRES1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-RARRES1 antibody
      See all RARRES1 primary antibodies
    • Description

      Rabbit polyclonal to RARRES1
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human RARRES1 aa 50-150 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab87814)

    • Positive control

      • Recombinant Human RARRES1 protein (ab114592) can be used as a positive control in WB. This antibody gave a positive signal in the following Whole cell lysates: HepG2; HEK293.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Retinoic & Retinoid
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab87115 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).

    Target

    • Sequence similarities

      Belongs to the protease inhibitor I47 (latexin) family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P49788 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5918 Human
      • Omim: 605090 Human
      • SwissProt: P49788 Human
      • Unigene: 131269 Human
      • Alternative names

        • 5430417P09Rik antibody
        • AI662122 antibody
        • RAR-responsive protein TIG1 antibody
        • RARRES1 antibody
        • Retinoic acid receptor responder (tazarotene induced) 1 antibody
        • Retinoic acid receptor responder protein 1 antibody
        • Tazarotene induced gene 1 protein antibody
        • Tazarotene-induced gene 1 protein antibody
        • TIG1 antibody
        • TIG1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-RARRES1 antibody (ab87115)
        Western blot - Anti-RARRES1 antibody (ab87115)
        All lanes : Anti-RARRES1 antibody (ab87115) at 1 µg/ml

        Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
        Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 33 kDa
        Observed band size: 40 kDa
        why is the actual band size different from the predicted?



        RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, the 40 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to RARRES1.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab87115? Please let us know so that we can cite the reference in this datasheet.

      ab87115 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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