Anti-RARS antibody (ab241085)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RARS antibody
See all RARS primary antibodies -
Description
Rabbit polyclonal to RARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RARS aa 1-50. The exact sequence is proprietary. (NP_002878.2)
Database link: P54136 -
Positive control
- WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as Arginyl tRNA synthetase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 75 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. -
Domain
The N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5917 Human
- Omim: 107820 Human
- SwissProt: P54136 Human
- Unigene: 654907 Human
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Form
There are 2 isoforms produced by alternative initiation. -
Alternative names
- 2610011N19Rik antibody
- 2610037E21Rik antibody
- AL033339 antibody
see all
Images
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Western blot - Anti-RARS antibody (ab241085)All lanes : Anti-RARS antibody (ab241085) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-RARS antibody (ab241085)RARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241085 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241085 at 0.4 µg/ml.
Lane 1: ab241085 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN buffer.
Datasheets and documents
References
ab241085 has not yet been referenced specifically in any publications.
