Product nameAnti-RARS antibody
See all RARS primary antibodies
DescriptionRabbit polyclonal to RARS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RARS aa 1-50. The exact sequence is proprietary. (NP_002878.2)
Database link: P54136
- WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as Arginyl tRNA synthetase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionForms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
DomainThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
- Information by UniProt
FormThere are 2 isoforms produced by alternative initiation.
- 2610011N19Rik antibody
- 2610037E21Rik antibody
- AL033339 antibody
All lanes : Anti-RARS antibody (ab241085) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
RARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241085 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241085 at 0.4 µg/ml.
Lane 1: ab241085 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN buffer.
ab241085 has not yet been referenced specifically in any publications.