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    rars-antibody-ab241085.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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Anti-RARS antibody (ab241085)

  • Datasheet
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Western blot - Anti-RARS antibody (ab241085)
  • Immunoprecipitation - Anti-RARS antibody (ab241085)

Key features and details

  • Rabbit polyclonal to RARS
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RARS protein (ab114873)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RARS antibody
    See all RARS primary antibodies
  • Description

    Rabbit polyclonal to RARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RARS aa 1-50. The exact sequence is proprietary. (NP_002878.2)
    Database link: P54136

  • Positive control

    • WB: HeLa, Jurkat and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

     This product was previously labelled as Arginyl tRNA synthetase

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human RARS protein (ab114873)
  • Related Products

    • Recombinant Human RARS protein (ab114873)

Applications

Our Abpromise guarantee covers the use of ab241085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
  • Domain

    The N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P54136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5917 Human
    • Omim: 107820 Human
    • SwissProt: P54136 Human
    • Unigene: 654907 Human
    • Form

      There are 2 isoforms produced by alternative initiation.
    • Alternative names

      • 2610011N19Rik antibody
      • 2610037E21Rik antibody
      • AL033339 antibody
      • Arginine tRNA ligase 1, cytoplasmic antibody
      • Arginine tRNA ligase antibody
      • Arginine--tRNA ligase antibody
      • Arginyl tRNA synthetase 1 antibody
      • Arginyl tRNA synthetase cytoplasmic antibody
      • Arginyl tRNA synthetase, cytoplasmic antibody
      • Arginyl-tRNA synthetase, cytoplasmic antibody
      • ArgRS antibody
      • AW985894 antibody
      • DALRD 1 antibody
      • DALRD1 antibody
      • EC 6.1.1.19 antibody
      • MGC8641 antibody
      • MGC86730 antibody
      • RARS antibody
      • SYRC_HUMAN antibody
      • wu:fa96g09 antibody
      • zgc:56050 antibody
      • zgc:86730 antibody
      see all

    Images

    • Western blot - Anti-RARS antibody (ab241085)
      Western blot - Anti-RARS antibody (ab241085)
      All lanes : Anti-RARS antibody (ab241085) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 75 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-RARS antibody (ab241085)
      Immunoprecipitation - Anti-RARS antibody (ab241085)

      RARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241085 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241085 at 0.4 µg/ml.

      Lane 1: ab241085 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

      Lysate prepared using NETN buffer.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241085? Please let us know so that we can cite the reference in this datasheet.

    ab241085 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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