Key features and details
- Rabbit polyclonal to Ras
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Ras antibody
See all Ras primary antibodies
DescriptionRabbit polyclonal to Ras
The immunogen used for this product shares 82% homology with HRAS and NRAS. Cross-reactivity with these proteins has not been confirmed experimentally.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: A-549, HeLa and Raji cell lysates. Mouse spleen, lung and rat brain tissue extracts. ICC/IF: C6 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 21 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
Involvement in diseaseDefects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
Cellular localizationCell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
- Information by UniProt
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
Immunofluorescence staining of C6 cells stained for RAS with ab180772 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-Ras antibody (ab180772) at 1/1000 dilution
Lane 1 : A-549 cell extract
Lane 2 : HeLa cell extract
Lane 3 : Raji cell extract
Lane 4 : Mouse spleen tissue extract
Lane 5 : Mouse lung tissue extract
Lane 6 : Rat brain tissue extract
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
ab180772 has been referenced in 14 publications.
- Wu YH et al. Comparing PI3K/Akt Inhibitors Used in Ovarian Cancer Treatment. Front Pharmacol 11:206 (2020). PubMed: 32194423
- Ren Y et al. CRNDE promotes cell tongue squamous cell carcinoma cell growth and invasion through suppressing miR-384. J Cell Biochem 120:155-163 (2019). PubMed: 30242873
- Chan K et al. eIF4A supports an oncogenic translation program in pancreatic ductal adenocarcinoma. Nat Commun 10:5151 (2019). PubMed: 31723131
- Wang CJ et al. The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs. Mol Cancer 18:115 (2019). PubMed: 31272462
- He F et al. Reversal of EGFR inhibitors' resistance by co-delivering EGFR and integrin avß3 inhibitors with nanoparticles in non-small cell lung cancer. Biosci Rep 39:N/A (2019). PubMed: 31316001