Key features and details
- Rabbit polyclonal to Ras
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ras antibody
See all Ras primary antibodies
DescriptionRabbit polyclonal to Ras
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Ras aa 31-43. The immunogen peptide is from the effector binding loop of the H, K and N-Ras.
Database link: P01116
- ICC/IF: HeLa and HaCaT cells. IHC: Mouse backskin tissue. WB: Mouse brain Cortex cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab240907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 22 kDa.|
FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
Involvement in diseaseDefects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
Cellular localizationCell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
- Information by UniProt
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
Anti-Ras antibody (ab240907) at 1/1000 dilution + Mouse brain Cortex cell lysate at 30 µg
Goat Anti-Rabbit IgG HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 5 minutes
Primary incubation for 2 hours at room temperature.
Block: 5% Skim Milk in 1X TBST
Bouin’s Fixative Solution-fixed Mouse backskin tissue stained for RAS (Green) using ab240907 at 1/100 dilution for 1 hours at RT in ICC/IF. FITC Goat Anti-Rabbit was used as the secondary antibody at 1/50 dilution for 1 hour at RT.
2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RAS (Red) using ab240907 at 1/200 dilution for 12 hours at 4°C. APC Goat Anti-Rabbit was used as the secondary antibody at 1:200 for 2 hours at RT. The nuclear counterstain was DAPI (Blue) at 1/40000 for 2 hours at RT.
2% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RAS (Green) using ab240907 at 1/200 dilution for 12 hours at 4ºC. FITC Goat Anti-Rabbit was used as the secondary antibody at 1/200 dilution for 2 hours at RT. The nuclear counterstain was DAPI (Blue) at 1/40000 dilution for 2 hours at RT.
Cold 100% Methanol-fixed HaCaT cells stained for RAS (Green) using ab240907 at 1/200 dilution for 12 hours at 4ºC in ICC/IF. Goat Anti-Rabbit was used as the secondary antibody at 1/50 dilution for 1-2 hours at RT in dark.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240907 has not yet been referenced specifically in any publications.