RecombinantRabMAb

Recombinant Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

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Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
  • Flow Cytometry - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
  • Immunoprecipitation - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
  • Western blot - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
  • Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3255] to Ras - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ras antibody [EPR3255] - BSA and Azide free
    See all Ras primary antibodies

  • Description

    Rabbit monoclonal [EPR3255] to Ras - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab108602 also detects H-Ras and N-Ras.

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Ras aa 1-100 (N terminal).
    Database link: P01112

  • Positive control

    • WB: 293T and SH-SY5Y cell lysates; Rat brain lysates; Mouse heart lysates IP: SH-SY5Y cell lysates Flow Cyt: HEK-293 cells ICC/IF: HEK-293T cells

  • General notes

    Ab209974 is the carrier-free version of ab108602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab209974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Function

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

  • Involvement in disease

    Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
    Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
    Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
    Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
    Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
    Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).

  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.

  • Post-translational
    modifications

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
    S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.

  • Cellular localization

    Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C BAS/HAS antibody
    • C HA RAS1 antibody
    • C-BAS/HAS antibody
    • c-H-ras antibody
    • C-HA-RAS1 antibody
    • CTLO antibody
    • GTPase HRas antibody
    • GTPase KRas antibody
    • GTPase NRas antibody
    • H ras antibody
    • H RASIDX antibody
    • H-Ras-1 antibody
    • H-RASIDX antibody
    • Ha-Ras antibody
    • HAMSV antibody
    • HRAS antibody
    • HRAS1 antibody
    • K ras antibody
    • K RAS2A antibody
    • K RAS2B antibody
    • K RAS4A antibody
    • K RAS4B antibody
    • K-RAS antibody
    • KRAS antibody
    • KRAS1 antibody
    • KRAS2 antibody
    • N-RAS antibody
    • N-terminally processed antibody
    • NRAS antibody
    • NRAS1 antibody
    • p21ras antibody
    • RASH_HUMAN antibody
    • RASH1 antibody
    • RASK2 antibody
    • Transforming protein p21 antibody
    • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
    • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti ras antibody epr3255 immunocytochemistry 293t human)
  • Flow Cytometry - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Flow Cytometry - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Ras with purified ab108602 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602)
  • Immunoprecipitation - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Immunoprecipitation - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    ab108602 (purified) at 1/20 dilution (2 µg) immunoprecipitating Ras in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab108602 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108602 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602)
  • Western blot - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Western blot - Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution (unpurified)

    Lane 1 : HEK-293 whole cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108602).

  • Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)
    Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab209974? Please let us know so that we can cite the reference in this datasheet.

ab209974 has not yet been referenced specifically in any publications.

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