Recombinant Anti-RASA1 antibody [EP536Y] (ab40677)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP536Y] to RASA1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RASA1 antibody [EP536Y]
See all RASA1 primary antibodies -
Description
Rabbit monoclonal [EP536Y] to RASA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human RASA1 aa 250-350 (internal sequence). The exact sequence is proprietary. From near the SH3 domain.
Database link: P20936 -
Positive control
- WB: Rat brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP536Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. -
Tissue specificity
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). -
Involvement in disease
Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5921 Human
- Entrez Gene: 218397 Mouse
- Entrez Gene: 25676 Rat
- Omim: 139150 Human
- SwissProt: P20936 Human
- SwissProt: P50904 Rat
- Unigene: 664080 Human
- Unigene: 12223 Rat
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Alternative names
- CM AVM antibody
- CMAVM antibody
- DKFZp434N071 antibody
see all
Images
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Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain tissue lysate at 10 µg
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cleavage fragment) -
Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab40677 has been referenced in 10 publications.
- Jiang LN et al. Identification of the circ_PRKDC/miR-20a-3p/RASA1 axis in regulating HaCaT keratinocyte migration. Wound Repair Regen 30:282-291 (2022). PubMed: 34897876
- Sun X et al. Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1. Mol Ther Nucleic Acids 24:164-174 (2021). PubMed: 33767913
- Cao Y et al. RNA-binding protein QKI suppresses breast cancer via RASA1/MAPK signaling pathway. Ann Transl Med 9:104 (2021). PubMed: 33569406
- Jing L et al. MicroRNA-4530 suppresses cell proliferation and induces apoptosis by targeting RASA1 in human umbilical vein endothelial cells. Mol Med Rep 19:3393-3402 (2019). PubMed: 30864691
- Kim DY et al. Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells. Oncol Lett 18:6361-6370 (2019). PubMed: 31814846
- Chen YL et al. Down-regulation of RASA1 Is Associated with Poor Prognosis in Human Hepatocellular Carcinoma. Anticancer Res 37:781-785 (2017). PubMed: 28179330
- Huang J et al. Co-expression and significance of Dok2 and Ras p21 protein activator 1 in breast cancer. Oncol Lett 14:5386-5392 (2017). PubMed: 29098030
- Teng HW et al. Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma. Sci Rep 6:35308 (2016). PubMed: 27752061
- Ying W et al. MicroRNA-223 is a crucial mediator of PPAR?-regulated alternative macrophage activation. J Clin Invest 125:4149-59 (2015). PubMed: 26436647
- Du C et al. Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma. J Exp Clin Cancer Res 34:67 (2015). WB, IHC ; Human . PubMed: 26126858